Transcription activation via enhanced preinitiation complex assembly in a human cell-free system lacking TAFIIs

Molecular Cell
T OelgeschlägerR G Roeder

Abstract

In contrast to previous findings in cell-free systems reconstituted with partially purified metazoan factors, we demonstrate dramatic activation of transcription in a TBP-dependent but TAFII-independent manner in HeLa nuclear extracts immunodepleted of TBP and major TAFIIs. Single-round transcription assays reveal that TAFII-independent activation is manifested at the level of productive preinitiation complex formation and that TAFIIs actually impair functional preinitiation complex assembly in a core promoter-specific manner. Furthermore, TAFIIs appear to elevate absolute levels of transcription under multiple-round transcription conditions, presumably by facilitating secondary initiation events. Finally, human coactivator activities related to those in yeast RNA polymerase II/mediator complexes appear to function in unfractionated HeLa nuclear extracts.

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Citations

Jun 6, 2000·Trends in Biochemical Sciences·S Malik, R G Roeder
Feb 8, 2006·Nature Structural & Molecular Biology·Brian A Lewis, Danny Reinberg
Jun 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·T KotaniT Kokubo
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·F C Holstege, R A Young
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·L C MyersR D Kornberg
Jul 23, 2013·Genes & Development·Wei-Yi ChenRobert G Roeder
Jul 20, 2002·Genes & Development·Kristina M JohnsonMichael Carey
Nov 14, 2000·Molecular and Cellular Biology·J M ParkY J Kim
Jan 3, 2001·Molecular and Cellular Biology·A Hernández-Hernández, A Ferrús
Feb 7, 2001·Molecular and Cellular Biology·M Mencía, K Struhl
Jul 26, 2002·Molecular and Cellular Biology·Steven L SandersP Anthony Weil
Oct 1, 2003·Molecular and Cellular Biology·Zheng Chen, James L Manley
Apr 3, 2004·Molecular and Cellular Biology·Shinya TakahataTetsuro Kokubo
Jan 20, 2005·Molecular and Cellular Biology·M M RobinsonL A Stargell
Apr 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mohamed OuhammouchE Peter Geiduschek
Jul 25, 2006·Critical Reviews in Biochemistry and Molecular Biology·Mary C Thomas, Cheng-Ming Chiang
Jun 9, 2004·European Journal of Biochemistry·Evelyn TamayoEdio Maldonado
Jul 14, 2004·Molecular and Cellular Endocrinology·Julián NevadoAna Aranda
May 18, 2005·Trends in Biochemical Sciences·Sohail Malik, Robert G Roeder
May 14, 1999·Current Opinion in Genetics & Development·M Hampsey, D Reinberg
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