PMID: 16632884Apr 25, 2006Paper

Transcription analysis of recombinant saccharomyces cerevisiae reveals novel responses to xylose

Applied Biochemistry and Biotechnology
Laura SalusjärviMerja Penttilä

Abstract

Lignocellulosic biomass, rich in hexose and pentose sugars, is an attractive resource for commercially viable bioethanol production. Saccharomyces cerevisiae efficiently ferments hexoses but is naturally unable to utilize pentoses. Metabolic engineering of this yeast has resulted in strains capable of xylose utilization. However, even the best recombinant S. cerevisiae strains of today metabolize xylose with a low rate compared to glucose. This study compares the transcript profiles of an S. cerevisiae strain engineered to utilize xylose via the xylose reductase-xylitol dehydrogenase pathway in aerobic chemostat cultures with glucose or xylose as the main carbon source. Compared to the glucose culture, 125 genes were upregulated, whereas 100 genes were downregulated in the xylose culture. A number of genes encoding enzymes capable of nicotinamide adenine dinucleotide phosphate regeneration were upregulated in the xylose culture. Furthermore, xylose provoked increased activities of the pathways of acetyl-CoA synthesis and sterol biosynthesis. Notably, our results suggest that cells metabolizing xylose are not in a completely repressed or in a derepressed state either, indicating that xylose was recognized neither as a fermentabl...Continue Reading

Citations

Jul 3, 2009·Applied Microbiology and Biotechnology·Akinori MatsushikaShigeki Sawayama
May 15, 2008·Applied Biochemistry and Biotechnology·Steven D BrownJonathan R Mielenz
Sep 6, 2008·Microbiology and Molecular Biology Reviews : MMBR·Elke Nevoigt
Jun 6, 2008·Microbial Cell Factories·Laura SalusjärviLaura Ruohonen
Sep 17, 2013·PloS One·Basti BergdahlMarie F Gorwa-Grauslund
Mar 26, 2013·Biotechnology Advances·Soo Rin KimJin-Ho Seo
Oct 7, 2015·Journal of Industrial Microbiology & Biotechnology·Akinori Matsushika, Tamotsu Hoshino
Jun 9, 2009·Yeast·Kaisa KarhumaaMarie-F Gorwa-Grauslund
Oct 13, 2016·Applied Microbiology and Biotechnology·Haibo LiHal S Alper
Mar 28, 2018·Nature Communications·Venkatesh Endalur Gopinarayanan, Nikhil U Nair
Oct 19, 2017·Applied Microbiology and Biotechnology·Laura SalusjärviLaura Ruohonen
Sep 2, 2018·Biotechnology Journal·Venkatesh Endalur Gopinarayanan, Nikhil U Nair
Apr 11, 2012·FEMS Yeast Research·Gionata ScalcinatiJens Nielsen
Nov 7, 2018·Applied Microbiology and Biotechnology·Joana T CunhaLucília Domingues
May 28, 2019·Microbial Cell Factories·Karen O OsiroMarie F Gorwa-Grauslund
Mar 16, 2021·Frontiers in Bioengineering and Biotechnology·Yixuan ZhuLimin Cao
Jun 8, 2021·Applied Microbiology and Biotechnology·B A BrandtR Den Haan

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