PMID: 6160021Jan 1, 1980Paper

Transcription and metabolism of RNA from the Drosophila melanogaster heat shock puff site 93D

Chromosoma
J A LengyelM L Pardue

Abstract

Characteristics of the major heat shock puff site 93D and the RNA transcribed from it have been investigated by hybridization to polytene chromosome preparations and to recombinant DNA. By saturation in situ hybridization, the length of the transcribed region at 93D is twice that of the mRNA coding region at the heat shock puff site 87A. From the known length of the heat shock mRNA sequence at 87A, we calculate that the minimum length of the transcribed region at 93D is 9.6 kb (kb = kilobase, i.e., 1,000 nucleotides).--The metabolism of RNA transcribed from 93D has been compared with that of RNA coding for the major heat shock protein hsp70 in cells incubated for one hour at 35 degrees C. Hsp70 mRNA sequences, assayed by hybridization to a specific recombinant DNA probe and by in situ hybridization to 87A, were found in both poly(A)+ and poly(A)- cytoplasmic RNA and were more concentrated in cytoplasmic RNA than in nuclear RNA. In contrast, sequences complementary to 93D, assayed by in situ hybridization, were more concentrated in nuclear than in cytoplasmic RNA. This implies that sequences from 93D exit from the nucleus at a lower rate and/or are turned over in the cytoplasm at a higher rate, than sequences from 87A. Site 93D ...Continue Reading

References

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Citations

Jan 1, 1984·Molecular & General Genetics : MGG·F P PetersB Hovemann
Jul 30, 2011·Journal of Biosciences·Subhash C Lakhotia
Jun 1, 1985·Developmental Biology·M RoarkJ A Lengyel
Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Y KawataH Ishikawa
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J L ZimmermanJ E Manning
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J C Garbe, M L Pardue
Nov 1, 1983·The Journal of Cell Biology·T Pederson
May 18, 2007·Annual Review of Cell and Developmental Biology·Mary-Lou Pardue
Jan 1, 1985·CRC Critical Reviews in Biochemistry·E A Craig
Jul 6, 2015·Biochimica Et Biophysica Acta·Timothy E Audas, Stephen Lee
Sep 15, 2012·Wiley Interdisciplinary Reviews. RNA·Subhash C Lakhotia
Jun 1, 1988·Journal of Cellular Physiology·S SadisL A Weber
Jan 1, 1988·Developmental Genetics·E M Underwood, J A Lengyel
Jan 1, 1988·Molecular and Cellular Biology·J BellM Pellegrini
Feb 1, 1983·Molecular and Cellular Biology·S Mayrand, T Pederson

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