PMID: 9171082Jun 15, 1997Paper

Transcription and polyadenylation in a short human intergenic region

Nucleic Acids Research
S BrackenridgeN J Proudfoot

Abstract

The poly(A) signal of the human Lamin B2 gene was previously shown to lie 600 bp upstream of the cap site of a gene of unknown function (ppv 1). However, using RNase protection analysis, we show that ppv 1 has two clusters of multiple initiation sites, so that the 5"cap site lies only approximately 280 nt downstream of the Lamin B2 poly(A) signal. We analysed nascent transcription across this unusually short intergenic region using nuclear run-on analysis of both the endogenous locus and of transiently transfected hybrid constructs. Surprisingly, transcription of the Lamin B2 gene does not appear to terminate prior to any of the mapped ppv 1 start sites, although pausing of the elongating polymerase complexes is observed downstream of the Lamin B2 poly(A) signal. We suggest that this pausing may be sufficient to protect the downstream gene from transcriptional interference. Finally, we have also investigated the sequences required for efficient recognition of the Lamin B2 poly(A) signal. We show that sequences upstream of the AAUAAA element are required for full activity, which is an unusual feature of mammalian poly(A) signals.

References

Sep 16, 1976·Nature·N J Proudfoot, G G Brownlee
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A ValsamakisJ C Alwine
Jan 1, 1989·Molecular and Cellular Biology·E WhitelawN J Proudfoot
Nov 1, 1989·Molecular and Cellular Biology·J D DeZazzo, M J Imperiale
Mar 1, 1989·Trends in Biochemical Sciences·N J Proudfoot
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L Conway, M Wickens
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·M GiaccaS Riva
May 1, 1996·Molecular and Cellular Biology·J BlauD Bentley

❮ Previous
Next ❯

Citations

Aug 10, 2010·The Journal of Biological Chemistry·Qiuming GongZhengfeng Zhou
Mar 25, 2000·Molecular and Cellular Biology·H Hans, J C Alwine
Oct 5, 2001·Molecular and Cellular Biology·D P TranH G Martinson
Jun 3, 2000·Journal of Molecular Biology·E de StanchinaG Biamonti
Dec 22, 1998·Molecular and Cellular Biology·C WilliamsT Blumenthal
Oct 3, 2017·Carcinogenesis·Chioniso P Masamha, Eric J Wagner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.