Transcription factor-associated combinatorial epigenetic pattern reveals higher transcriptional activity of TCF7L2-regulated intragenic enhancers

BMC Genomics
Qi LiuVictor X Jin

Abstract

Recent studies have suggested that combinations of multiple epigenetic modifications are essential for controlling gene expression. Despite numerous computational approaches have been developed to decipher the combinatorial epigenetic patterns or "epigenetic code", none of them has explicitly addressed the relationship between a specific transcription factor (TF) and the patterns. Here, we developed a novel computational method, T-cep, for annotating chromatin states associated with a specific TF. T-cep is composed of three key consecutive modules: (i) Data preprocessing, (ii) HMM training, and (iii) Potential TF-states calling. We evaluated T-cep on a TCF7L2-omics data. Unexpectedly, our method has uncovered a novel set of TCF7L2-regulated intragenic enhancers missed by other software tools, where the associated genes exert the highest gene expression. We further used siRNA knockdown, Co-transfection, RT-qPCR and Luciferase Reporter Assay not only to validate the accuracy and efficiency of prediction by T-cep, but also to confirm the functionality of TCF7L2-regulated enhancers in both MCF7 and PANC1 cells respectively. Our study for the first time at a genome-wide scale reveals the enhanced transcriptional activity of cell-typ...Continue Reading

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Citations

Apr 3, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Merit L GoodmanChristy R Hagan
Feb 26, 2021·BioMed Research International·Xiaotong DongYunxiang Zhang
May 11, 2021·BioMed Research International·Lan YuDejun Sun

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Methods Mentioned

BETA
ChIP-seq
RNA-seq
PCR
Assay
transfection

Software Mentioned

ChomHMM
intersectBed
HMMSeg
manyOutputMapper
Epigenomics
Virtual bin Creation
ChromaSig
OpenMP
Bowtie
RepeatMasker

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