PMID: 9191024May 1, 1997Paper

Transcription factor C/EBPalpha: novel sites of expression and cloning of the human gene

Biological Chemistry
G W SwartJ Schalkwijk

Abstract

We describe the characterization of recombinant clones for the human transcription factor CCAAT/enhancer binding protein alpha (hC/EBP alpha). The intronless hC/EBP alpha gene is almost 90% homologous to its rat and mouse counterparts. The gene copies of more distant species are less conserved, but the alignment reveals a striking homology in five regions, of which four may be involved in transactivation functions while the fifth concerns the carboxy-terminal bZip sequences (basic region and leucine zipper) mediating sequence specific DNA-binding. In addition to the usual expression sites, significant transcript levels were detected in the epidermal compartment of human skin and in rat aorta by northern analysis. The presence of hC/EBP alpha is further documented by immunohistochemical analysis of human skin biopsies and cultured keratinocytes showing the nuclear presence of the protein, notably in the suprabasal layers of the epidermis and in human keratinocytes induced to differentiate.

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Citations

May 4, 2001·Journal of Immunological Methods·K Theilgaard-MönchN Borregaard
Aug 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Daniel HelblingThomas Pabst
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·C MüllerA Leutz
Dec 2, 2004·Molecular and Cellular Biology·Kyungsil Yoon, Robert C Smart
Apr 18, 2006·Biochimica Et Biophysica Acta·Mikkel Bruhn Schuster, Bo Torben Porse
Apr 15, 2000·The Journal of Biological Chemistry·Y L Zhou, M L Snead
Sep 13, 2014·Journal of Hematology & Oncology·Friederike PastoreKarsten Spiekermann
Dec 9, 2003·The Journal of Biological Chemistry·Christine MüllerAchim Leutz

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