Transcription factor E2F and cyclin E-Cdk2 complex cooperate to induce chromosomal DNA replication in Xenopus oocytes.

The Journal of Biological Chemistry
E AkamatsuJ Y Kato

Abstract

Although no chromosomal DNA replication actually occurs during Xenopus oocyte maturation, the capability develops during the late meiosis I (MI) phase in response to progesterone. This ability, however, is suppressed by Mos proteins and maturation/mitosis promoting factor during the second meiosis phase (meiosis II; MII) until fertilization. Inhibition of RNA synthesis by actinomycin D during early MI prevented induction of the replication ability, but did not interfere with initiation of the meiotic cell cycle progression characterized by oscillation of the maturation/mitosis promoting factor activity and germinal vesicle breakdown. Microinjection of recombinant proteins such as dominant-negative E2F or universal Cdk inhibitors, p21 and p27, but not wild type human E2F-1 or Cdk4-specific inhibitor, p19, into maturing oocytes during MI abolished induction of the DNA replication ability. Co-injection of human E2F-1 and cyclin E proteins into immature oocytes allowed them to initiate DNA replication even in the absence of progesterone treatment. Injection of cyclin E alone, which was sufficient to activate endogenous Cdk2 kinase, failed to induce DNA replication. Moreover, the activation of Cdk2 was not affected under the conditi...Continue Reading

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Citations

Sep 13, 2012·Nature Communications·Masatoshi HaraTakeo Kishimoto
Dec 2, 2010·Molecular Biology of the Cell·Grace S TanKatrina F Cooper
Apr 15, 1999·The EMBO Journal·J L LenormandD J Donoghue
Sep 3, 2003·Cell Structure and Function·Toshiaki TanakaJun-ya Kato
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·J TianJ V Ruderman
Aug 3, 2006·The Journal of Biological Chemistry·Wonkyung OhJaewhan Song
Feb 29, 2000·The Journal of Biological Chemistry·Y ArataJ Y Kato
May 29, 2002·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·S Wadler

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