Transcription factor motif enrichment in whole transcriptome analysis identifies STAT4 and BCL6 as the most prominent binding motif in systemic juvenile idiopathic arthritis

Arthritis Research & Therapy
Boris HügleKlaus Tenbrock

Abstract

The term systemic juvenile idiopathic arthritis (sJIA) describes an autoinflammatory condition characterized by arthritis and severe systemic inflammation, which in later stages can transform into interleukin (IL)-17-driven autoimmune arthritis. IL-1 antagonists have been used with good efficacy in the early stages of sJIA. A whole transcriptome analysis of peripheral blood RNA samples was performed in six patients with sJIA and active systemic disease, before initiating treatment with the IL-1β receptor antagonist anakinra, and after induction of inactive disease, compared with a single-sample control cohort of 21 patients in several clinical stages of sJIA activity. Whole transcriptomes were compared longitudinally and interindividually including gene ontology and motif enrichment analysis of differentially expressed genes. There were 741 transcripts were identified using a threshold with a p value <0.01 and a fold change > 2. HLADRB1 and CD74 were identified as the most strongly upregulated genes in inactive compared to active disease; CD177 expression was significantly enhanced in active disease compared to inactive disease. Motif enrichment analysis revealed STAT4, BCL6, and STAT3 as the most prominent transcription factor...Continue Reading

References

Jun 24, 2000·Transfusion·K MatsuoD Stroncek
May 22, 2001·Clinical and Experimental Immunology·S Kerlan-CandonJ F Eliaou
May 30, 2003·The Biochemical Journal·Peter C HeinrichFred Schaper
Mar 31, 2004·Journal of Translational Medicine·David F StroncekMaria Bettinotti
Feb 8, 2006·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Gordon A AwandareDouglas J Perkins
Dec 16, 2006·Annals of the Rheumatic Diseases·Wilco de JagerBerent J Prakken
Aug 29, 2007·The Journal of Experimental Medicine·Florence AllantazVirginia Pascual
Jul 1, 2009·Arthritis and Rheumatism·Michael G BarnesRobert A Colbert
Oct 24, 2009·Molecular Neurodegeneration·Shuji MatsudaLuciano D'Adamio
Jan 12, 2010·Current Opinion in Rheumatology·Joseph Holoshitz
Mar 3, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Behnaz BayatSentot Santoso
Jun 8, 2011·Nature Reviews. Rheumatology·Elizabeth D MellinsAlexei A Grom
Jul 1, 2011·Arthritis Care & Research·Carol A WallaceUNKNOWN Paediatric Rheumatology International Trials Organisation
Nov 24, 2011·International Journal of Biological Sciences·Cesar A TerrazasMiriam Rodriguez-Sosa
Jun 13, 2012·Annals of the Rheumatic Diseases·Joachim GerssUNKNOWN Paediatric Rheumatology International Trials Organization (PRINTO)
May 25, 2013·Nucleic Acids Research·Jing WangBing Zhang
Feb 15, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Konstantia-Maria ChaveleMichael R Ehrenstein
Nov 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Michael J OmbrelloUNKNOWN International Childhood Arthritis Genetics (INCHARGE) Consortium
Dec 17, 2015·Pediatric Rheumatology Online Journal·Christiane S EberhardtKlaus Tenbrock
Jan 25, 2017·Arthritis Research & Therapy·Arndt H BrachatUNKNOWN Pediatric Rheumatology International Trials Organization (PRINTO) and the Pediatric Rheumatology Collaborative Study Group (

❮ Previous
Next ❯

Citations

Jan 29, 2019·Modern Rheumatology·Shinji Akioka
May 17, 2019·Nature Reviews. Rheumatology·Robert BuschElizabeth D Mellins
Mar 7, 2021·Journal of Clinical Medicine·Stylianos TomarasEugen Feist
Sep 2, 2021·Clinical & Translational Immunology·Samira ImranRichard Saffery

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
PCR
reverse transcription PCR
flow cytometry

Software Mentioned

GeneSpring GX
WebGestalt
Affymetrix® Expression ConsoleTM
AltAnalyze
MotifMatch
SPSS

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

B cell Activation

B cell activation is initiated by the ligation of the B cell receptor with antigen and ultimately results in the production of protective antibodies against potentially pathogenic invaders. Here is the latest research.