Transcription factor VdCmr1 is required for pigment production, protection from UV irradiation, and regulates expression of melanin biosynthetic genes in Verticillium dahliae

Microbiology
Yonglin WangSteven J Klosterman

Abstract

Verticillium dahliae is a soilborne fungus that causes vascular wilt diseases on numerous plant species worldwide. The production of darkly melanized microsclerotia is crucial in the disease cycle of V. dahliae, as these structures allow for long-term survival in soil. Previously, transcriptomic and genomic analysis identified a cluster of genes in V. dahliae that encodes some dihydroxynaphthalene (DHN) melanin biosynthetic pathway homologues found in related fungi. In this study, we explored the roles of cluster-specific transcription factor VdCmr1, as well as two other genes within the cluster encoding a polyketide synthase (VdPKS1) and a laccase (VdLac1), enzymes at initial and endpoint steps in DHN melanin production. The results revealed that VdCmr1 and VdPKS1 are required for melanin production, but neither is required for microsclerotia production. None of the three genes were required for pathogenesis on tobacco and lettuce. Exposure of ΔVdCmr1 and wild-type strains to UV irradiation, or to high temperature (40 °C), revealed an approx. 50 % reduction of survival in the ΔVdCmr1 strain, relative to the wild-type strain, in response to either condition. Expression profiles revealed that expression of some melanin biosynthe...Continue Reading

References

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Oct 24, 2008·Phytopathology·Qing-Ming QinKrishna V Subbarao
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Sep 11, 2013·BMC Genomics·Dechassa DuressaSteven J Klosterman
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Citations

Jun 18, 2020·Plants·Nuria Montes-Osuna, Jesús Mercado-Blanco
Oct 28, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Xiang LiYuan Li
May 12, 2021·Molecular Plant Pathology·Evan JohnKar-Chun Tan
Apr 24, 2021·Annual Review of Phytopathology·Jie-Yin ChenKrishna V Subbarao

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Methods Mentioned

BETA
PCR
Assay
gene knockout

Software Mentioned

REST
JMP
mega

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