Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata

Frontiers in Microbiology
Jing WuLiming Liu

Abstract

Candida glabrata is an important microorganism used in commercial fermentation to produce pyruvate, but very little is known about its mechanisms for surviving acid stress in culture. In this study, it was shown that transcription factors Asg1p and Hal9p play essential roles in C. glabrata in the tolerance of acid stress, as the deletion of CgASG1 or CgHAL9 resulted in the inability to survive in an acidic environment. Cgasg1Δ and Cghal9Δ mutant strains are unable to maintain pH homeostasis, as evidenced by a decrease in intracellular pH and an increase in reactive oxygen species production, which results in metabolic disorders. The results showed that intracellular acidification was partly due to the diminished activity of the plasma membrane proton pump, CgPma1p. In addition, transcriptome sequencing revealed that Cgasg1Δ and Cghal9Δ mutant strains displayed a variety of changes in gene expression under acidic conditions, including genes in the MAPK signaling pathway, plasma membrane, or cell wall organization, trehalose accumulation, and the RIM101 signaling pathway. Lastly, quantitative reverse-transcribed PCR and cellular localization showed that CgAsg1p and CgHal9p played independent roles in response to acid stress.

References

Apr 29, 1998·FEBS Letters·I MendizabalI F de Larrinoa
May 20, 1999·The Journal of Cell Biology·F MadeoK U Fröhlich
Dec 11, 1999·Nucleic Acids Research·A KumarM Snyder
May 23, 2001·Nucleic Acids Research·B AkacheB Turcotte
Oct 11, 2003·Biotechnology Advances·K Schügerl
Apr 5, 2007·Nature Protocols·Tania NolanStephen A Bustin
Jan 11, 2008·Proteomics·Pia SchmidtAlistair J P Brown
Feb 27, 2008·Biochimica Et Biophysica Acta·Gabriel G PerroneIan W Dawes
Feb 18, 2010·Journal of Bioscience and Bioengineering·Siraje Arif MahmudHiroshi Shimizu
Apr 3, 2012·Medical Mycology·Despoina KaloritiAlistair J P Brown
May 28, 2013·Metabolic Engineering·Xiulai ChenJian Chen
Nov 26, 2013·Revista iberoamericana de micología·Marcela Briones-Martin-Del-CampoAlejandro De Las Peñas
Dec 11, 2013·Frontiers in Microbiology·Zeljkica JandricChristoph Schüller

❮ Previous
Next ❯

Citations

Jun 1, 2016·Frontiers in Microbiology·Jawad MerhejFrédéric Devaux
May 20, 2018·Applied and Environmental Microbiology·Hui LiuLiming Liu
Jul 2, 2017·Applied and Environmental Microbiology·Xiaobao LinLiming Liu
Sep 25, 2016·Applied and Environmental Microbiology·Dongni YanJian Chen
Apr 5, 2020·Applied and Environmental Microbiology·Chengjin WuXiulai Chen
Mar 28, 2017·Applied Microbiology and Biotechnology·Zhengshan LuoJian Chen
Aug 27, 2021·Journal of Fungi·Yahaya HassanLeslie Thian Lung Than

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Fluorescence
RNA Extraction
fluorescence microscopy
RNAseq
protein folding
reverse-transcribed PCR

Related Concepts

Related Feeds

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.