Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression.

BMC Molecular Biology
Aneta KaszaJolanta Jura

Abstract

MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Here we report that the proinflammatory cytokine IL-1beta rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1beta. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1beta stimulation.

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Citations

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Methods Mentioned

BETA
sumoylation
acetylation
histone acetylation
PMA
PCR
transfection
immunoprecipitation
FCS
ChIP-chip
ChIP

Software Mentioned

MCPIP

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