Transcription factors Ets2 and Sp1 act synergistically with histone acetyltransferase p300 in activating human interleukin-12 p40 promoter

Acta Biochimica Et Biophysica Sinica
Haijing SunBaiqu Huang

Abstract

There has been considerable interest in researching the regulatory mechanisms that control the synthesis of interleukin (IL)-12, which plays a central role in the differentiation of T-helper-1 cells. In this study, we performed a series of transient transfection experiments designed to elucidate the functional relationship between the IL-12 promoter-specific transcription factors (Ets2 and Sp1) and histone acetylation modification in IL-12 regulation mediated by p300 and various histone deacetylases (HDACs). Results presented in this report demonstrated that the transcription factors Ets2 and Sp1 acted synergistically with p300 to activate the human IL-12 promoter. The histone acetyltransferase (HAT) activity of p300 was required for this synergic effect, because the adenovirus E1A protein inhibited the synergy. Conversely, HDACs repressed the synergic effect of transcription factors and histone acetylation on the activation of IL-12, while p300 was able to rectify it. These data indicated that Ets2 and Sp1 worked concertedly and synergistically with p300 in the regulation of human IL-12 expression.

References

May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·U GublerP C Familletti
Oct 1, 1995·Molecular and Cellular Biology·T L MurphyK M Murphy
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·M E GerritsenT Collins
Sep 29, 1999·Genes & Development·R E Kingston, G J Narlikar
Jan 29, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T SuzukiM Horikoshi
Mar 30, 2001·The Journal of Biological Chemistry·B R KumarT K Kundu
May 8, 2001·The Journal of Biological Chemistry·R L EricksonO A MacDougald
Aug 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Gregg L Semenza
Nov 14, 2002·The Biochemical Journal·Annemieke J M de RuijterAndré B P van Kuilenburg
Nov 16, 2004·Journal of Cellular Biochemistry·Haijing SunBaiqu Huang
Mar 9, 2005·Archives of Biochemistry and Biophysics·Yangguang ShaoBaiqu Huang

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Citations

Dec 3, 2015·PLoS Neglected Tropical Diseases·Michelle A FavilaMary Ann McDowell
Jun 20, 2020·Communications Biology·Saadia KhiljiQiao Li
Jan 5, 2011·Immunological Reviews·Luigi Buonaguro, Bali Pulendran
Jun 13, 2020·Circulation Research·Wilson Lek Wen TanUNKNOWN CHARGE-Heart Failure Working Group, CHARGE-EchoGen Consortium
Dec 3, 2008·The Journal of Experimental Medicine·Denis GaucherRafick-Pierre Sékaly
Mar 16, 2011·FEBS Letters·Ann Florence B VictorianoTakashi Okamoto
Dec 9, 2020·Viruses·Antoine DutilleulCarine Van Lint
Dec 2, 2008·Biochimica Et Biophysica Acta·Xitong DangKlaus Ley
Sep 29, 2011·Seminars in Cell & Developmental Biology·Stryder M MeadowsPaul A Krieg

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