Transcription factors Rv0081 and Rv3334 connect the early and the enduring hypoxic response of Mycobacterium tuberculosis

Virulence
Xian SunYao Li

Abstract

The ability of Mycobacterium tuberculosis (M. tb) to survive and persist in the host for decades in an asymptomatic state is an important aspect of tuberculosis pathogenesis. Although adaptation to hypoxia is thought to play a prominent role underlying M. tb persistence, how the bacteria achieve this goal is largely unknown. Rv0081, a member of the DosR regulon, is induced at the early stage of hypoxia while Rv3334 is one of the enduring hypoxic response genes. In this study, we uncovered genetic interactions between these two transcription factors. RNA-seq analysis of ΔRv0081 and ΔRv3334 revealed that the gene expression profiles of these two mutants were highly similar. We also found that under hypoxia, Rv0081 positively regulated the expression of Rv3334 while Rv3334 repressed transcription of Rv0081. In addition, we demonstrated that Rv0081 formed dimer and bound to the promoter region of Rv3334. Taken together, these data suggest that Rv0081 and Rv3334 work in the same regulatory pathway and that Rv3334 functions immediately downstream of Rv0081. We also found that Rv3334 is a bona fide regulator of the enduring hypoxic response genes. Our study has uncovered a regulatory pathway that connects the early and the enduring hy...Continue Reading

References

Sep 1, 1977·Infection and Immunity·L G Wayne
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·D R ShermanG K Schoolnik
Sep 7, 2001·Annual Review of Microbiology·L G Wayne, C D Sohaskey
Nov 26, 2002·Journal of Bacteriology·Calvin Boon, Thomas Dick
Dec 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lanbo ShiRobert J North
Jun 28, 2003·FEMS Microbiology Reviews·Nigel L BrownJon L Hobman
Sep 4, 2003·The Journal of Experimental Medicine·Dirk SchnappingerGary K Schoolnik
Sep 4, 2003·The Journal of Experimental Medicine·Martin I VoskuilGary K Schoolnik
Dec 13, 2003·Tuberculosis·James E Gomez, John D McKinney
Mar 23, 2004·Annual Review of Immunology·Robert J North, Yu-Jin Jung
Mar 24, 2004·Nature Reviews. Microbiology·Graham R StewartDouglas B Young
Sep 9, 2004·The Journal of Experimental Medicine·Petros C KarakousisWilliam R Bishai
Dec 21, 2004·Nature Reviews. Microbiology·Helena I M Boshoff, Clifton E Barry
Apr 12, 2005·Annual Review of Pharmacology and Toxicology·Ying Zhang
Dec 5, 2006·Nature Reviews. Microbiology·Kim Lewis
Mar 29, 2007·PLoS Medicine·Lynn E ConnollyLalita Ramakrishnan
Sep 14, 2007·Immunological Reviews·Kyle RohdeDavid G Russell
Jan 31, 2008·PloS One·Tige R RustadDavid R Sherman
Mar 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Amit K Pandey, Christopher M Sassetti
Mar 19, 2008·Infection and Immunity·Laura E ViaClifton E Barry
May 14, 2008·Cell Host & Microbe·Michael U ShilohJeffery S Cox
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Apr 9, 2009·Journal of Cell Science·Carine WhiteMichael J Petris
Apr 25, 2009·Cellular Microbiology·Tige R RustadDavid R Sherman
Jun 3, 2009·Infection and Immunity·Ryan W HonakerMartin I Voskuil
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Laith J Abu-RaddadM Elizabeth Halloran
Mar 4, 2010·Genome Biology·Mark D Robinson, Alicia Oshlack
Jul 20, 2010·Cell Host & Microbe·David G RussellKyle H Rohde
Sep 10, 2010·Annual Review of Microbiology·Michael C Chao, Eric J Rubin
Mar 25, 2011·Mucosal Immunology·J L FlynnP L Lin
Jul 5, 2013·Nature·James E GalaganGary K Schoolnik
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
RNA-seq
electrophoretic mobility shift

Software Mentioned

HTSeq
Bowtie2
Seqtk
edgeR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.