Transcription factors SOHLH1 and SOHLH2 coordinate oocyte differentiation without affecting meiosis I

The Journal of Clinical Investigation
Yong-Hyun ShinAleksandar Rajkovic

Abstract

Following migration of primordial germ cells to the genital ridge, oogonia undergo several rounds of mitotic division and enter meiosis at approximately E13.5. Most oocytes arrest in the dictyate (diplotene) stage of meiosis circa E18.5. The genes necessary to drive oocyte differentiation in parallel with meiosis are unknown. Here, we have investigated whether expression of spermatogenesis and oogenesis bHLH transcription factor 1 (Sohlh1) and Sohlh2 coordinates oocyte differentiation within the embryonic ovary. We found that SOHLH2 protein was expressed in the mouse germline as early as E12.5 and preceded SOHLH1 protein expression, which occurred circa E15.5. SOHLH1 protein appearance at E15.5 correlated with SOHLH2 translocation from the cytoplasm into the nucleus and was dependent on SOHLH1 expression. NOBOX oogenesis homeobox (NOBOX) and LIM homeobox protein 8 (LHX8), two important regulators of postnatal oogenesis, were coexpressed with SOHLH1. Single deficiency of Sohlh1 or Sohlh2 disrupted the expression of LHX8 and NOBOX in the embryonic gonad without affecting meiosis. Sohlh1-KO infertility was rescued by conditional expression of the Sohlh1 transgene after the onset of meiosis. However, Sohlh1 or Sohlh2 transgene expr...Continue Reading

References

Oct 5, 2002·Nucleic Acids Research·Laura A Cassiday, L James Maher
Jun 24, 2004·The Journal of Comparative Neurology·Hitoshi YamataniTatsumi Hirata
Aug 25, 2004·Science·Aleksandar RajkovicMartin M Matzuk
Oct 30, 2004·Genesis : the Journal of Genetics and Development·Laura A BannisterJohn C Schimenti
Nov 18, 2005·Developmental Biology·Allison E FalenderJoanne S Richards
Mar 28, 2006·Developmental Biology·D BallowA Rajkovic
May 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stephanie A PangasAleksandar Rajkovic
Jun 13, 2006·Gene Expression Patterns : GEP·Daniel J BallowAleksandar Rajkovic
Apr 12, 2007·PLoS Biology·Laura A BannisterJohn C Schimenti
May 15, 2007·Biology of Reproduction·Youngsok ChoiAleksandar Rajkovic
Jun 7, 2007·Genesis : the Journal of Genetics and Development·Teresa GallardoDiego H Castrillon
Jun 15, 2007·BMC Developmental Biology·Saurabh JoshiJurrien Dean
Jul 13, 2007·Fertility and Sterility·Yingying QinAleksandar Rajkovic
May 30, 2008·Biology of Reproduction·Youngsok ChoiAleksandar Rajkovic
Nov 19, 2008·Developmental Biology·Shuichi ToyodaJun-ichi Miyazaki
Mar 18, 2009·Bioinformatics·Cole TrapnellSteven L Salzberg
Apr 12, 2011·Nature Cell Biology·Katrin DanielAttila Tóth
Nov 8, 2011·Developmental Biology·Hitomi SuzukiAleksandar Rajkovic
Jun 19, 2013·Nature Genetics·Gregoriy A DokshinDavid C Page
Aug 15, 2013·Human Molecular Genetics·Marcy E RichardsonMiles F Wilkinson
Feb 15, 2014·Fertility and Sterility·Yingying QinZi-Jiang Chen
Aug 8, 2014·PLoS Genetics·Jana KoubovaDavid C Page
Mar 17, 2015·The Journal of Clinical Endocrinology and Metabolism·Yavuz BayramJames R Lupski
Jul 5, 2016·Oncogene·A-S TournillonR Fåhraeus

❮ Previous
Next ❯

Citations

Oct 26, 2018·Molecular Reproduction and Development·Gongqing LiuZhihong Zheng
Sep 17, 2018·Journal of Assisted Reproduction and Genetics·Deepika Delsa DeanPoonam Tripathi
Mar 10, 2019·Journal of the American Society of Nephrology : JASN·Szu-Yuan LiKatalin Susztak
Mar 19, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jingjing ShiJiang Fu
May 12, 2019·Biology of Reproduction·Svetlana A Yatsenko, Aleksandar Rajkovic
Apr 18, 2018·EMBO Molecular Medicine·Marie Christou-KentChristophe Arnoult
Nov 11, 2017·Biology of Reproduction·Xinye WangAleksandar Rajkovic
May 16, 2017·The Journal of Clinical Investigation·T Rajendra Kumar
Dec 21, 2019·PLoS Genetics·Eliska TaborskaPetr Svoboda
May 19, 2020·The Journal of Reproduction and Development·Maria SorrentiMassimo DE Felici
Nov 26, 2020·Journal of Cellular and Molecular Medicine·Maria Giovanna DesimioDonatella Farini
Dec 18, 2020·Nature·Nobuhiko HamazakiKatsuhiko Hayashi
Apr 21, 2019·Molecular Cell·Michiel BoekhoutScott Keeney
Apr 25, 2021·Ageing Research Reviews·F Timóteo-FerreiraE Silva
May 25, 2021·Current Opinion in Endocrine and Metabolic Research·Jessica M O'Connell, Melissa E Pepling
Jun 23, 2021·Aging·Svetlana YurenevaGennady Sukhikh

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
nuclear translocation
GEO profile
RNA-seq
PCR

Software Mentioned

Cufflinks
Nobox
TopHat
Cuffdiff

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Stephanie A PangasAleksandar Rajkovic
Gene Expression Patterns : GEP
Daniel J BallowAleksandar Rajkovic
Clinical and Experimental Reproductive Medicine
Eun-Jin Lim, Youngsok Choi
© 2022 Meta ULC. All rights reserved