Transcription factors underlying wing margin color patterns and pupal cuticle markings in butterflies

EvoDevo
Robert D ReedC R Brunetti

Abstract

The diversity of butterfly color patterns can be attributed to a relatively small number of pattern elements that are homologous across Lepidoptera. Although genes involved in patterning some of these elements have been identified, the development of several major elements remains poorly understood. To identify genes underlying wing pupal cuticle markings and wing margin color patterns, we examined expression of the candidate transcription factors Engrailed/Invected (En/Inv), Distal-less (Dll), Cubitus interruptus (Ci), and Spalt in two nymphalids: Junonia coenia and Bicyclus anynana. We found that En/Inv, Dll, and Ci mark domains on the J. coenia last-instar forewing disc that closely correspond to the position and shape of pupal cuticle markings. We also found that Spalt demarcates wing margin color patterns in both J. coenia and B. anynana, and that CRISPR/Cas9 deletions in the spalt gene result in reduction and loss of wing margin color patterns in J. coenia. These data demonstrate a role for spalt in promoting wing margin color patterning, in addition to its previously described role in eyespot patterning. Our observations support the model that a core set of regulatory genes are redeployed multiple times, and in multiple ...Continue Reading

References

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Nov 13, 2019·Proceedings of the National Academy of Sciences of the United States of America·James J LewisRobert D Reed

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Spalt

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