PMID: 8955315Dec 1, 1996Paper

Transcription-independent DNA translocation of bacteriophage T7 DNA into Escherichia coli

Journal of Bacteriology
L R García, I J Molineux

Abstract

Penetration of wild-type T7 DNA into the host cell occurs in two steps. The phage particle ejects a few hundred base pairs of the left end of the genome into the host. Translocation of the remainder of the DNA is then coupled to transcription. In a normal infection, transcription-coupled translocation of wild-type T7 DNA is initiated at the major A1, A2, and A3 promoters for Escherichia coli RNA polymerase. At 37 degrees C, various deletion mutants lacking these three promoters grow at the same efficiency as wild-type T7 because the minor B promoter is efficiently transferred from the phage head into the cell. As the temperature of the phage infection decreases, the latent periods of (A1, A2, A3)- phages increase relative to that of wild-type T7; nevertheless, (A1, A2, A3)- phages have normal plating efficiencies at reduced temperatures. Lengthening of the latent period at low temperatures is due to a delay in transferring the complete (A1, A2, A3)- genome into the host cell. The (A1, A2, A3)- phages eject the leading end of their genome into the host, but at low temperature, insufficient DNA is transferred into the cell to allow RNA polymerase immediate access the B promoter. However, by an inefficient transcription-independen...Continue Reading

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Citations

Jun 6, 2009·Molecular Biology and Evolution·Thomas E KellerJames J Bull
Jan 24, 2014·Nanoscale·Mercedes Hernando-PérezPedro J de Pablo
May 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·D EndyI J Molineux
Jun 30, 2007·Evolution; International Journal of Organic Evolution·Richard H Heineman, James J Bull
Apr 15, 2018·Journal of Biological Physics·Pedro J de PabloJosé L Carrascosa
Jul 18, 2018·PeerJ·Eric F TomJames J Bull
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·L R García, I J Molineux
Nov 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·J KindtW M Gelbart
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Sep 15, 2021·Open Biology·Sari MäntynenSamuel L Díaz-Muñoz
Jun 8, 2001·Analytical Chemistry·J J KasianowiczB Robertson

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