PMID: 9444993Jan 28, 1998Paper

Transcription mapping of the Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) genome in a body cavity-based lymphoma cell line (BC-1)

Journal of Virology
R SaridP S Moore

Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV) gene transcription in the BC-1 cell line (KSHV and Epstein-Barr virus coinfected) was examined by using Northern analysis with DNA probes extending across the viral genome except for a 3-kb unclonable rightmost region. Three broad classes of viral gene transcription have been identified. Class I genes, such as those encoding the v-cyclin, latency-associated nuclear antigen, and v-FLIP, are constitutively transcribed under standard growth conditions, are unaffected by tetradecanoylphorbol acetate (TPA) induction, and presumably represent latent viral transcripts. Class II genes are primarily clustered in nonconserved regions of the genome and include small polyadenylated RNAs (T0.7 and T1.1) as well as most of the virus-encoded cytokines and signal transduction genes. Class II genes are transcribed without TPA treatment but are induced to higher transcription levels by TPA treatment. Class III genes are primarily structural and replication genes that are transcribed only following TPA treatment and are presumably responsible for lytic virion production. These results indicate that BC-1 cells have detectable transcription of a number of KSHV genes, particularly nonconserved genes inv...Continue Reading

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Citations

Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·N DupinC Boshoff
Oct 20, 2000·The Journal of General Virology·K T HallA Whitehouse
Apr 20, 2001·Journal of Virology·M Paulose-MurphyS Zeichner
Jun 26, 2001·International Journal of Experimental Pathology·C Swanton, N Jones
Apr 26, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Thomas B CampbellLovemore Gwanzura
May 29, 2003·The Journal of General Virology·Charles CunninghamAndrew J Davison
Jun 10, 2003·Microbiology and Molecular Biology Reviews : MMBR·Lyubomir A DourmishevDavid M Lukac
Sep 13, 2003·Journal of Virology·DeeAnn Martinez-GuzmanRen Sun
Dec 6, 2003·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Miriam MargalithDharam V Ablashi
Apr 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Xuezhong CaiBryan R Cullen
Aug 19, 2010·Immunologic Research·Ting-Ting WuRen Sun

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