Transcription-mediated amplification (TMA) for the assessment of viremia in hemodialysis patients with hepatitis C

Journal of Medical Virology
Dauana O BastosMaria Lucia Ferraz

Abstract

The diagnosis of hepatitis C virus (HCV) infection in hemodialysis patients is difficult particularly due to the presence of intermittent viremia. The aims of this study were: (a) to determine the prevalence of intermittent viremia in hemodialysis patients with anti-HCV antibodies who tested negative for HCV RNA by PCR at the first evaluation and (b) to evaluate the contribution of the transcription-mediated amplification method (TMA) to the diagnosis of viremia in the PCR-negative samples. One hundred and six patients with anti-HCV antibodies and an initial negative result for HCV RNA by PCR were included. An additional sample was collected for a second HCV RNA test by PCR after a minimum interval of 3 months and a positive result characterized intermittent viremia. HCV RNA was investigated by TMA in the PCR-negative sample of patients with intermittent viremia, and in the most recent sample from patients with PCR-negative results in both determinations. Intermittent viremia was observed in 60/106 (57%) patients (57% men; age: 45 ± 10 years). Fifty-one of the 60 negative samples from patients with intermittent viremia and 29/46 double-negative patients were tested by TMA. This assay detected viremia in 20/51 (39%) samples of i...Continue Reading

References

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Citations

Jan 17, 2015·World Journal of Gastroenterology : WJG·Roberto J Carvalho-FilhoMaria Lucia G Ferraz
Feb 7, 2017·The International Journal of Artificial Organs·Pavlina Dzekova-Vidimliski, Aleksandar Sikole
Feb 27, 2019·Jornal brasileiro de nefrologia : ʹorgão oficial de Sociedades Brasileira e Latino-Americana de Nefrologia·Natasha Silva ConstancioMarcelo Mazza do Nascimento

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