PMID: 8602358Feb 1, 1996Paper

Transcription-mediated binding of peptide nucleic acid (PNA) to double-stranded DNA: sequence-specific suicide transcription

Nucleic Acids Research
H J Larsen, P E Nielsen

Abstract

Peptide nucleic acid (PNA) forms sequence-specific (PNA)2/DNA triplexes with one strand of double-stranded DNA by strand invasion. When formed with the template strand of DNA such a (PNA)2/DNA triplex can arrest transcription elongation in vitro and can thus act as an anti-gene agent. One of the major obstacles to applying PNA as an anti-gene agent in vivo is that PNA strand invasion occurs at a very slow rate under moderate salt conditions. In the present study we show that transcription can increase the rate of sequence-specific PNA binding dramatically. Such transcription-mediated PNA binding occurs three times as efficiently when the PNA target is situated on the non- template strand as compared with the template strand. Since transcription can mediate template strand-associated (PNA)2/DNA complexes which arrest further elongation, the action of RNA polymerase results in repression of its own activity, i.e. suicide transcription. These findings are highly relevant for the possible future use of PNA as an anti-gene agent.

References

Nov 27, 1992·Science·J C HanveyK G Au
Dec 11, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L J Maher
Aug 1, 1995·Current Opinion in Biotechnology·Y Choo, A Klug
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·V V DemidovP E Nielsen
Feb 1, 1993·Current Opinion in Biotechnology·C Hélène
Sep 1, 1994·Journal of Molecular Recognition : JMR·P E NielsenO Buchardt
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·N E MøllegaardP E Nielsen
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·N J PefferL E Babiss
Jan 25, 1993·Nucleic Acids Research·P E NielsenO Buchardt
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D Y ChernyP E Nielsen
May 1, 1995·Journal of Peptide Science : an Official Publication of the European Peptide Society·L ChristensenR H Berg

❮ Previous
Next ❯

Citations

Feb 19, 2003·Journal of Molecular Biology·Irina V SmolinaMaxim D Frank-Kamenetskii
Feb 27, 1999·Current Opinion in Biotechnology·P E Nielsen
Apr 30, 2004·Cell Research·Gan Wang, Xiaoxin S Xu
Jun 21, 2002·Antisense & Nucleic Acid Drug Development·Uffe KoppelhusPeter Eigil Nielsen
Aug 1, 1997·Antisense & Nucleic Acid Drug Development·L Good, P E Nielsen
Mar 25, 2005·Oligonucleotides·Amy J ZiembaScot W Ebbinghaus
Jun 13, 2006·Oligonucleotides·S Bøe, E Hovig
Dec 25, 2007·Oligonucleotides·Zhaoyang YeRam I Mahato
Aug 23, 2000·Nucleic Acids Research·X ZhangD R Corey
Oct 21, 2006·Nucleic Acids Research·Thomas BentinPeter E Nielsen
Aug 5, 2000·Applied and Environmental Microbiology·D P ChandlerF J Brockman
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·A F FaruqiP M Glazer
Dec 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·Faye A RogersPeter M Glazer
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·J LohseP E Nielsen
Jan 13, 2006·Nature Chemical Biology·Bethany A JanowskiDavid R Corey
Jan 13, 2006·Nature Chemical Biology·Bruce A Armitage
Feb 15, 2001·Expert Opinion on Investigational Drugs·P E Nielsen
Dec 1, 1996·Annals of Medicine·H Lönnberg, E Vuorio
Sep 9, 2015·Archives of Biochemistry and Biophysics·Douglas IversonAnthony J Bell
Mar 24, 2009·Molecular Carcinogenesis·Boris P BelotserkovskiiPhilip C Hanawalt
Aug 22, 2013·Protein Science : a Publication of the Protein Society·Filbert Totsingan, Anthony J Bell
Apr 17, 2010·Chemistry & Biodiversity·Peter E Nielsen
Jun 11, 1999·Current Opinion in Structural Biology·P E Nielsen
Jun 26, 2004·Chemistry & Biology·Kunihiro KaihatsuDavid R Corey
Mar 13, 1998·Chemistry & Biology·A KurakinP E Nielsen
Apr 28, 2004·Biophysical Journal·Ayome AbibiMaxim D Frank-Kamenetskii
Jun 1, 2006·Advances in Genetics·Karin E LundinC I Edvard Smith
Oct 28, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shinjiro SawadaKunihiro Kaihatsu
Nov 14, 2000·The Journal of Biological Chemistry·G DieciS Ottonello
Sep 23, 2006·Organic & Biomolecular Chemistry·Ramin HamzaviNils Metzler-Nolte
Nov 11, 2003·The Journal of Biological Chemistry·Laurence VaysseCharles Coutelle
Nov 26, 2002·The Journal of Biological Chemistry·Monica BorgattiRoberto Gambari
Dec 1, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S DiviaccoC Giovannangeli
Jun 2, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Ray, B Nordén
Oct 21, 2003·Nucleosides, Nucleotides & Nucleic Acids·Xin ZhaoDavid R Corey
Apr 3, 2021·International Journal of Biological Macromolecules·Yusuke HamashitaToshihiko Sakurai
Aug 12, 2004·Journal of the American Chemical Society·Simon FichtOliver Seitz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved