PMID: 4901068Nov 1, 1969Paper

Transcription of bacteriophage T4 deoxyribonucleic acid in vitro

The Biochemical Journal
J O Bishop, F W Robertson

Abstract

1. RNA was synthesized in vitro from a template of bacteriophage T4 DNA, in the presence of Mn(2+). A comparison was made of the RNA synthesized by purified RNA polymerase from two sources, Micrococcus lysodeikticus and Escherichia coli; these are referred to as Micrococcus cRNA and E. coli cRNA respectively (where cRNA indicates RNA synthesized in vitro by using purified RNA polymerase and a DNA primer). 2. Both types of RNA were self-complementary as judged by resistance to digestion with ribonuclease after self-annealing, Micrococcus cRNA being more self-complementary (40%) than was E. coli cRNA (30%). The cRNA was found to be much less self-complementary if Mg(2+) was present during RNA synthesis instead of Mn(2+). 3. Micrococcus cRNA hybridized with a larger part of bacteriophage T4 DNA than did E. coli cRNA. The E. coli cRNA competed with only part (70%) of the Micrococcus cRNA in hybridization-competition experiments. It is concluded that more sequences of bacteriophage T4 DNA are transcribed by Micrococcus polymerase than by E. coli polymerase. 4. The RNA sequences synthesized by Micrococcus RNA polymerase but not by E. coli RNA polymerase are shown by hybridization competition to compete with specifically late bacterio...Continue Reading

Citations

Jan 1, 1973·Chromosoma·D E Graham, D M Skinner
Sep 25, 1972·European Journal of Biochemistry·R M Hecht, M L Birnstiel
Jul 13, 1972·European Journal of Biochemistry·M MeilhacP Chambon
Oct 22, 1972·Life Sciences. Pt. 2: Biochemistry, General and Molecular Biology·J J FurthK Fujii

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