Transcription of [FeFe]-Hydrogenase Genes during H2 Production in Clostridium and Desulfovibrio spp. Isolated from a Paddy Field Soil

Microbes and Environments
Ryuko BabaTakeshi Watanabe

Abstract

Changes in the relative abundances of the transcripts of hydA gene paralogs for [FeFe]-hydrogenase in Clostridium sp. strain H2 and Desulfovibrio sp. strain A1 isolated from paddy field soil were analyzed during H2 production. Strains H2 and A1 had at least five and two phylogenetically different hydA genes, respectively. The relative abundances of their hydA transcripts differed among the paralogs and H2 production activity changed in a manner that depended on the growth phase and conditions. Increases or decreases in the relative abundances of the transcripts of two out of five hydA genes in strain H2 correlated with changes in H2 production rates, whereas those of the others remained unchanged or decreased. In strain A1, the relative abundances of the transcripts of two hydA genes differed between monoculture, sulfate-reducing, and syntrophic, methanogenic conditions. The relative abundance of the transcripts of one hydA gene, predicted to encode a cytosolic [FeFe]-hydrogenase, was higher under syntrophic, methanogenic conditions than sulfate-reducing conditions, while that of the transcripts of the other hydA gene decreased with time under both conditions. This study showed that the transcription of the hydA gene during gro...Continue Reading

References

Jun 1, 1980·Journal of Bacteriology·N L Schauer, J G Ferry
Jan 21, 2000·FEMS Microbiology Ecology·K Glissmann, R Conrad
Aug 29, 2001·FEMS Microbiology Reviews·P M VignaisJ Meyer
Oct 7, 2004·Applied and Environmental Microbiology·Tillmann LuedersMichael W Friedrich
Mar 14, 2007·Cellular and Molecular Life Sciences : CMLS·J Meyer
Oct 12, 2007·Chemical Reviews·Paulette M Vignais, Bernard Billoud
Jul 8, 2009·Journal of Bacteriology·Christopher B WalkerDavid A Stahl
Feb 2, 2010·Applied and Environmental Microbiology·Oliver SchmidtMarcus A Horn
Apr 17, 2010·Microbiology·Magdalena CalusinskaAnnick Wilmotte
Apr 5, 2011·Applied and Environmental Microbiology·Pengfei LiuYahai Lu
Jul 13, 2011·Frontiers in Microbiology·Inês A Cardoso PereiraSofia Santos Venceslau
Jun 26, 2012·Nucleic Acids Research·Andreas UntergasserSteven G Rozen
Jul 19, 2012·Annual Review of Microbiology·Jessica R SieberRobert P Gunsalus
Dec 25, 2012·Journal of Bacteriology·Birte MeyerDavid A Stahl
Dec 3, 2014·Biochimica Et Biophysica Acta·John W PetersMichael W W Adams

❮ Previous
Next ❯

Datasets Mentioned

BETA
AB302951
LC194779
LC194785

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

R
Excel
EMBOSS Transeq
R for
Primer3Plus
R package ‘ DTK ’
ClustalW

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.