Transcription of laminin gamma1 chain gene in rat mesangial cells: constitutive and inducible RNA polymerase II recruitment and chromatin states

American Journal of Physiology. Renal Physiology
Joel D NelsonKarol Bomsztyk

Abstract

The laminin gamma1 chain, a critical component of the extracellular matrix, is encoded by the 125-kb-long Lamc1 locus. We profiled RNA polymerase II (Pol II) and histone modifications along the Lamc1 locus to explore transcription of this gene in its native chromatin environment. Treatment with 12-O-tetradecanoylphorbol-13-acetate increased Lamc1 mRNA in rat mesangial cells (RMC). This increase was matched by an increase in Pol II density along the entire length of the Lamc1 locus. In contrast, in the hepatocarcinoma cell line (HTC-IR) an increase in Pol II density was restricted to the promoter and was not followed by mRNA induction. The pattern of histone H3 methylation was similar for both cell types but an increase in H3 lysine 9 acetylation observed at the 5'-end was weaker in HTC-IR cells than in RMC. All of the histone modifications showed spatial patterns where levels differed greatly between the 5'- and 3'-ends of Lamc1. Conversely, at the short, highly induced egr-1 gene the differences in chromatin marks between the 5'- and 3'-ends were much smaller. The results of this study suggest that 1) Lamc1 transcription can be controlled after transcription initiation, to our knowledge, the first time this has been shown in a...Continue Reading

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Citations

Apr 1, 2011·Journal of Developmental Origins of Health and Disease·O DenisenkoS Bagby
Dec 14, 2012·American Journal of Physiology. Renal Physiology·Hang YuanRama Natarajan
Dec 25, 2009·American Journal of Physiology. Renal Physiology·Richard A Zager, Ali C M Johnson
Jun 27, 2009·Journal of the American Society of Nephrology : JASN·Masayo NaitoKarol Bomsztyk
Oct 4, 2013·Kidney International·Marpadga A ReddyRama Natarajan
May 12, 2015·Critical Care : the Official Journal of the Critical Care Forum·Karol BomsztykOleg Denisenko
Mar 20, 2013·Laboratory Investigation; a Journal of Technical Methods and Pathology·Radko KomersKarol Bomsztyk

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