PMID: 8948499Aug 1, 1996Paper

Transcription of melanogenesis enzymes in melanocytes: dependence upon culture conditions and co-cultivation with keratinocytes

Pigment Cell Research
S KippenbergerH Holzmann

Abstract

Eumelanogenesis of human skin melanocytes requires at least three enzymes: tyrosinase, TRP 1, and TRP 2. The regulation of these enzymes on transcriptional level was detected in a semiquantitative attempt. The total RNA of melanocytes was reverse-transcripted and followed by a PCR with degenerated primers for all three enzymes. The amplification products were related to each other densitometrically. We examined five different culture conditions: 1) melanocytes in a popular phorbolester containing F-10-medium, 2) melanocytes in a co-culture medium with EGF, 3) melanocytes in a co-culture medium with high calcium, 4) melanocytes co-cultured with keratinocytes in EGF containing co-culture medium, and 5) melanocytes co-cultured with keratinocytes in co-culture medium with high calcium. Melanocytes cultured in phorbolester containing F-10-medium featured transcripts of tyrosinase, TRP 1, and TRP 2 in the ratio 45:45:10. The same results were obtained for melanocytes co-cultured with keratinocytes under the two different culture conditions. In melanocytes cultured alone in co-culture media only TRP 1-transcripts were present. It is likely that under co-culture conditions a keratinocyte-derived factor supports the transcription of all...Continue Reading

References

May 11, 1992·FEBS Letters·A K ChakrabortyJ M Pawelek
Jun 25, 1991·Proceedings of the National Academy of Sciences of the United States of America·C D ChintamaneniB S Kwon
Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B C TrapnellR G Crystal
May 11, 1990·Nucleic Acids Research·T CohenS Shibahara
Apr 1, 1990·The Journal of Experimental Medicine·S VijayasaradhiA N Houghton
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B S KwonR Halaban
Jan 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·R HalabanA Baird
Oct 1, 1988·Medicinal Research Reviews·G Prota
Jan 1, 1987·The International Journal of Biochemistry·V J Hearing, M Jiménez
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·I J Jackson
Aug 1, 1983·The Journal of Cell Biology·R HalabanA B Lerner
Oct 1, 1995·Journal of the American Academy of Dermatology·J M GrichnikM G Hitchcock
Aug 1, 1994·Pigment Cell Research·T KobayashiV J Hearing
Nov 15, 1994·Biochemical and Biophysical Research Communications·F SolanoJ A Lozano
Dec 1, 1993·Journal of Dermatological Science·H Y Park, B A Gilchrest
May 1, 1994·The Journal of Clinical Investigation·E SchauerT A Luger
Aug 1, 1993·Pigment Cell Research·K UrabeV J Hearing
Jan 11, 1994·Biochimica Et Biophysica Acta·C SalinasJ A Lozano
Feb 1, 1993·PCR Methods and Applications·M M BlanchardV Nowotny

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Citations

Nov 28, 2002·Pigment Cell Research·Christine DuvalRainer Schmidt
Nov 6, 2007·Biological & Pharmaceutical Bulletin·Masago IshikawaFumio Ishii
Jul 31, 2008·Journal of Enzyme Inhibition and Medicinal Chemistry·Szu-Hsiu LiuI-Horng Pan
Mar 8, 2003·Pigment Cell Research·Tae-Jin Yoon, Vincent J Hearing
Apr 2, 2008·International Journal of Cosmetic Science·Z WangT Zhang
Jan 8, 2008·Experimental Dermatology·Karin U SchallreuterJennifer D Spencer
Jul 16, 2005·Journal of Photochemistry and Photobiology. B, Biology·Vincent K M PoonAndrew Burd
Mar 25, 2016·International Journal of Molecular Sciences·Naira V MargaryanLuigi Strizzi
Aug 2, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christopher G TuckerBrian T Fife
Jun 11, 2002·Analytical Biochemistry·Tie Chi LeiVincent J Hearing

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