PMID: 6171771Oct 10, 1981Paper

Transcription of T-DNA in octopine and nopaline crown gall tumours is inhibited by low concentrations of alpha-amanitin

Nucleic Acids Research
L WillmitzerJ Schell

Abstract

Highly purified and physiologically active nuclei were isolated from four different octopine and nopaline crown gall lines. These nuclei exhibited a high endogenous RNA synthesizing activity involving all three RNA-polymerases I, II and III. Isolated nuclei were shown by Southern blotting to synthesize T-DNA specific RNA. This synthesis was shown to be sensitive to actinomycin D and therefore to be DNA-dependent. The transcription of the T-DNA was also inhibited for more than 90% by low concentrations of alpha-amanitin (0.7 micrograms/ml) indicating that the T-DNA, although from bacterial origin, is transcribed by the host RNA polymerase II.

References

Apr 11, 1979·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J SchellR Villarroel
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J L ManleyM L Gefter
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·W B GurleyJ Callis
Jul 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·L RymoC Weissmann
Aug 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M JacquetJ Hurwitz
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R WeinmannR G Roeder
Jun 15, 1974·Journal of Molecular Biology·I ZaenenJ Schell
Apr 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·D B Clewell, D R Helinski
Sep 19, 1980·Science·P ZambryskiH M Goodman
Jan 1, 1981·Molecular & General Genetics : MGG·L WillmitzerJ Schell

❮ Previous
Next ❯

Citations

Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J LogemannL Willmitzer
Jan 25, 1983·Nucleic Acids Research·M BevanM D Chilton
Oct 25, 1984·Nucleic Acids Research·C H ShawC H Shaw
May 1, 1996·The Plant Cell·S B NarasimhuluS B Gelvin
Jun 5, 2007·Biochimica Et Biophysica Acta·Stanton B Gelvin, Sang-Ic Kim
Aug 12, 2006·Canadian Journal of Physiology and Pharmacology·Benoît LacroixVitaly Citovsky
Mar 1, 1992·Microbiological Reviews·S C Winans
Jun 1, 1986·Microbiological Reviews·L J Halverson, G Stacey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.