Transcription of the human cytomegalovirus genome in productively infected cells

The Journal of General Virology
C C ChuaS St Jeor

Abstract

Nuclear and cytoplasmic RNAs, synthesized in cells productively infected with human cytomegalovirus (HCMV) were analysed at various times after infection by liquid and filter DNA-RNA hybridization. Results of these experiments have revealed that: (i) the fraction of the genome transcribed increased as infection progressed. In the nucleus, transcripts represented approx. 20% of the virus DNA sequences at both 2 and 4 h post-infection (p.i.) and 36% of the virus DNA at 40 h p.i; (ii) the increase in virus sequences among nuclear transcripts at late times was prevented by the DNA synthesis inhibitor, 2'-deoxyfluorouridine; (iii) early virus RNA transcripts were subset of those represented in late RNA; (iv) two classes of early RNA were identified by competition hybridization; (v) approx. 10% of the late nuclear transcripts were symmetrical. Results of filter hybridization at DNA excess indicated that virus-specific RNA represented 0.6% of RNA labelled from 0 to 2 h p.i., and 1.8% of RNA labelled from 28 to 30 h. Polyadenylated RNA isolated from cytoplasm represented 1.2% and 10% of labelled mRNA at 2 h and 30 h respectively. Our data show that during productive infection of human cells by HCMV, gene expression is under temporal, q...Continue Reading

Citations

Dec 29, 2000·Microbiology and Immunology·E S HwangC Y Cha
Dec 1, 1988·Transfusion Medicine Reviews·E S Mocarski
Mar 16, 2012·Annals of the New York Academy of Sciences·Andrew J Davison
Sep 1, 1988·Virology·A M Colberg-Poley, L D Santomenna
May 1, 1990·Journal of Virology·L D Santomenna, A M Colberg-Poley
Jan 1, 1984·Journal of Virology·R M StenbergM F Stinski

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