PMID: 9422585Jan 9, 1998Paper

Transcription of the Rhodobacter sphaeroides cycA P1 promoter by alternate RNA polymerase holoenzymes

Journal of Bacteriology
B J MacGregorT J Donohue

Abstract

These experiments sought to identify what form of RNA polymerase transcribes the P1 promoter for the Rhodobacter sphaeroides cytochrome c2 gene (cycA). In vitro, cycA P1 was recognized by an RNA polymerase holoenzyme fraction that transcribes several well-characterized Escherichia coli heat shock (sigma32) promoters. The in vivo effects of mutations flanking the transcription initiation site (+1) also suggested that cycA P1 was recognized by an RNA polymerase similar to E. coli Esigma32. Function of cycA P1 was not altered by mutations more than 35 bp upstream of position +1 or by alterations downstream of -7. A point mutation at position -34 that is towards the E. coli Esigma32 -35 consensus sequence (G34T) increased cycA P1 activity approximately 20-fold, while several mutations that reduced or abolished promoter function changed highly conserved bases in presumed -10 or -35 elements. In addition, cycA P1 function was retained in mutant promoters with a spacer region as short as 14 nucleotides. When either wild-type or G34T promoters were incubated with reconstituted RNA polymerase holoenzymes, cycA P1 transcription was observed only with samples containing either a 37-kDa subunit that is a member of the heat shock sigma fact...Continue Reading

References

Jun 1, 1992·Journal of Bacteriology·M LonettoC A Gross
Jul 1, 1991·Journal of Bacteriology·B J MacGregor, T J Donohue
Jan 1, 1991·Methods in Enzymology·T J Donohue, S Kaplan
May 25, 1989·Nucleic Acids Research·R KarlsW S Reznikoff
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J E Stefano, J D Gralla
Apr 1, 1995·Journal of Bacteriology·B A Schilke, T J Donohue
Jan 9, 1998·Journal of Bacteriology·R K KarlsT J Donohue

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Citations

Sep 11, 1999·FEMS Microbiology Letters·J Gregor, G Klug
Nov 2, 2004·Journal of Bacteriology·Stephan BraatschMark Gomelsky
Aug 4, 2006·Journal of Bacteriology·Heather A Green, Timothy J Donohue
Apr 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jennifer R AnthonyTimothy J Donohue
Jan 9, 1998·Journal of Bacteriology·R K KarlsT J Donohue

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