Sep 11, 2012

Transcription of two long noncoding RNAs mediates mating-type control of gametogenesis in budding yeast

Cell
Folkert J van WervenAngelika Amon

Abstract

The cell-fate decision leading to gametogenesis is essential for sexual reproduction. In S. cerevisiae, only diploid MATa/α but not haploid MATa or MATα cells undergo gametogenesis, known as sporulation. We find that transcription of two long noncoding RNAs (lncRNAs) mediates mating-type control of sporulation. In MATa or MATα haploids, expression of IME1, the central inducer of gametogenesis, is inhibited in cis by transcription of the lncRNA IRT1, located in the IME1 promoter. IRT1 transcription recruits the Set2 histone methyltransferase and the Set3 histone deacetylase complex to establish repressive chromatin at the IME1 promoter. Inhibiting expression of IRT1 and an antisense transcript that antagonizes the expression of the meiotic regulator IME4 allows cells expressing the haploid mating type to sporulate with kinetics that are indistinguishable from that of MATa/α diploids. Conversely, expression of the two lncRNAs abolishes sporulation in MATa/α diploids. Thus, transcription of two lncRNAs governs mating-type control of gametogenesis in yeast.

  • References43
  • Citations98

Citations

Mentioned in this Paper

SMN1 gene
Pathologic Cytolysis
Saccharomyces cerevisiae Proteins
Northern Blotting
Biochemical Pathway
Nucleosomes
RGR1
HIRA gene
Amonafide
Deoxyribonuclease I

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