PMID: 3757031Sep 26, 1986Paper

Transcription of vaccinia virus early genes by enzymes isolated from vaccinia virions terminates downstream of a regulatory sequence

Cell
G RohrmannB Moss

Abstract

We describe an in vitro transcription system in which the polyadenylated 3' ends of vaccinia virus mRNAs are formed by termination downstream of a regulatory signal. When linear DNA templates containing vaccinia early genes were incubated with soluble enzymes extracted from vaccinia virions, mature-size mRNAs were synthesized within 1 min, whereas longer run-off transcripts were not detected until later. Deleting a series of thymidylate residues located downstream of the coding region abolished termination. The 3' ends of the transcripts formed heterogeneously, between 50 and 70 nucleotides past the T-rich termination signal, in either viral- or vector-derived sequences. Polyadenylation of the transcripts occurred without regard to the terminal sequence and was inhibited by adding exogenous RNA. Although exogenous RNAs were also polyadenylated, processing of 3' ends was not observed.

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Citations

Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L YuenB Moss
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S K Arya, R C Gallo
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P D Gershon, B Moss
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J B AntczakD J Pickup
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·C Lu, R Bablanian
Jan 30, 2010·The Journal of Biological Chemistry·Sean David TaylorAnna Marie Pyle
Sep 12, 2000·Journal of Virology·J Jin, L A Guarino
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L Yuen, B Moss
Mar 12, 2013·The Journal of Biological Chemistry·Nicole E KaySusan M D'Costa
Apr 25, 2008·Virology·Sarah PiacenteEdward G Niles
Jun 23, 2004·Virology·Mohamed Ragaa Mohamed, Edward G Niles
Feb 8, 2006·Virology·Mohamed Ragaa MohamedEdward G Niles
Feb 19, 1998·Journal of Molecular Biology·T K Biswas, G S Getz
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·J P Richardson
Jun 7, 2008·Canadian Journal of Microbiology·Heather E EatonCraig R Brunetti
Mar 30, 2001·The Journal of Biological Chemistry·M R Mohamed, E G Niles

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