Transcription, processing and function of CRISPR cassettes in Escherichia coli.

Molecular Microbiology
Ksenia PougachKonstantin Severinov

Abstract

CRISPR/Cas, bacterial and archaeal systems of interference with foreign genetic elements such as viruses or plasmids, consist of DNA loci called CRISPR cassettes (a set of variable spacers regularly separated by palindromic repeats) and associated cas genes. When a CRISPR spacer sequence exactly matches a sequence in a viral genome, the cell can become resistant to the virus. The CRISPR/Cas systems function through small RNAs originating from longer CRISPR cassette transcripts. While laboratory strains of Escherichia coli contain a functional CRISPR/Cas system (as judged by appearance of phage resistance at conditions of artificial co-overexpression of Cas genes and a CRISPR cassette engineered to target a λ-phage), no natural phage resistance due to CRISPR system function was observed in this best-studied organism and no E. coli CRISPR spacer matches sequences of well-studied E. coli phages. To better understand the apparently 'silent'E. coli CRISPR/Cas system, we systematically characterized processed transcripts from CRISPR cassettes. Using an engineered strain with genomically located spacer matching phage λ we show that endogenous levels of CRISPR cassette and cas genes expression allow only weak protection against infecti...Continue Reading

References

Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Mar 9, 2002·Omics : a Journal of Integrative Biology·Rund JansenLeo M Schouls
Apr 16, 2002·Molecular Microbiology·Ruud JansenLeo M Schouls
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Thean-Hock TangAlexander Hüttenhofer
Mar 29, 2005·Journal of Molecular Evolution·Francisco J M MojicaElena Soria
Jun 14, 2006·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Masanari KitagawaHirotada Mori
Aug 1, 2006·Archaea : an International Microbiological Journal·Reidun K LillestølKim Brügger
Mar 24, 2007·Science·Rodolphe BarrangouPhilippe Horvath
Dec 11, 2007·Journal of Bacteriology·Philippe HorvathRodolphe Barrangou
Dec 11, 2007·Journal of Bacteriology·Hélène DeveauSylvain Moineau
Aug 16, 2008·Science·Ryland F Young
Aug 16, 2008·Science·Stan J J BrounsJohn van der Oost
Sep 2, 2008·Journal of Bacteriology·Kristine B ArnvigCatherine L Squires
Dec 20, 2008·Science·Luciano A Marraffini, Erik J Sontheimer
May 23, 2009·FEMS Microbiology Letters·Ekaterina SemenovaKonstantin Severinov
Sep 30, 2009·Journal of Molecular Biology·Haeyoung JeongJihyun F Kim
Dec 1, 2009·Cell·Caryn R HaleMichael P Terns
Jan 9, 2010·Science·Philippe Horvath, Rodolphe Barrangou
Jan 15, 2010·Nature·Luciano A Marraffini, Erik J Sontheimer
Feb 6, 2010·Microbiology·C Díez-VillaseñorF J M Mojica
May 1, 2010·Microbiology·C Díez-VillaseñorF J M Mojica

❮ Previous
Next ❯

Citations

Jul 12, 2012·Nature Communications·Kirill A DatsenkoEkaterina Semenova
May 17, 2011·Nature Structural & Molecular Biology·Emily M GesnerAndrew M Macmillan
May 17, 2011·Nature Structural & Molecular Biology·Dipali G SashitalJennifer A Doudna
Dec 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Asma Hatoum-AslanLuciano A Marraffini
Nov 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ido YosefUdi Qimron
Aug 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ido YosefUdi Qimron
Mar 15, 2011·Journal of Bacteriology·Kyle C Cady, George A O'Toole
Jun 8, 2012·Molekuliarnaia biologiia
May 16, 2013·PLoS Genetics·Olga A SoutourinaIsabelle Martin-Verstraete
Feb 6, 2014·PLoS Neglected Tropical Diseases·Laetitia FabreFrançois-Xavier Weill
Jun 1, 2011·PloS One·Connor T SkennertonGene W Tyson
May 5, 2012·PloS One·Daan C SwartsStan J J Brouns
Nov 29, 2012·PloS One·Cristóbal AlmendrosFrancisco J M Mojica
Mar 19, 2013·Annual Review of Biochemistry·Rotem SorekBlake Wiedenheft
Mar 1, 2014·Bioinformatics·Ambarish BiswasChris M Brown
Nov 9, 2011·Annual Review of Genetics·Devaki BhayaRodolphe Barrangou
Jan 10, 2012·Annual Review of Food Science and Technology·Rodolphe Barrangou, Philippe Horvath
Nov 14, 2012·Annual Review of Genetics·Edze R WestraJohn van der Oost
Apr 15, 2014·Nucleic Acids Research·Sergey ShmakovKonstantin Severinov
Jun 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ekaterina SemenovaKonstantin Severinov
Nov 21, 2015·Nucleic Acids Research·Daria VorontsovaEkaterina Semenova
Nov 21, 2015·Nucleic Acids Research·Chaoyou XueDipali G Sashital
Mar 8, 2016·Molecular Cell·Samuel H SternbergUdi Qimron
Sep 24, 2010·Molecular Microbiology·Francisco J M Mojica, César Díez-Villaseñor
Mar 23, 2013·Wiley Interdisciplinary Reviews. RNA·Rodolphe Barrangou
Jan 12, 2011·Molecular Microbiology·Mohan BabuAlexander F Yakunin
May 9, 2014·Molecular Microbiology·Robert HelerDavid Bikard
Sep 10, 2015·Nucleic Acids Research·Srivatsa DwarakanathLennart Randau
Oct 10, 2015·Environmental Science and Pollution Research International·Sabine DelannoyPatrick Fach
Feb 22, 2014·Trends in Genetics : TIG·Rebecca M Terns, Michael P Terns

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.