Transcription profiling associated with life cycle of Anopheles gambiae

Journal of Medical Entomology
Brent W HarkerFrank H Collins

Abstract

Complex biological events occur during the developmental process of the mosquito Anopheles gambiae (Giles). Using cDNA expression microarrays, the expression patterns of 13,440 clones representing 8,664 unique transcripts were revealed from six different developmental stages: early larvae (late third instar/early fourth instar), late larvae (late fourth instar), early pupae (< 30 min after pupation), late pupae (after tanning), and adult female and male mosquitoes (24 h postemergence). After microarray analysis, 560 unique transcripts were identified to show at least a fourfold up- or down-regulation in at least one developmental stage. Based on the expression patterns, these gene products were clustered into 13 groups. In total, eight genes were analyzed by quantitative real-time polymerase chain reaction to validate microarray results. Among 560 unique transcripts, 446 contigs were assigned to respective genes from the An. gambiae genome. The expression patterns and annotations of the genes in the 13 groups are discussed in the context of development including metabolism, transport, protein synthesis and degradation, cellular processes, cellular communication, intra- or extra-cellular architecture maintenance, response to str...Continue Reading

Citations

Jul 15, 1981·Developmental Biology·L S Levy, J E Manning
Nov 1, 1995·Experimental Parasitology·Liangbiao ZhengFotis C Kafatos
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L F AppelJ Fristrom
Sep 1, 1996·Genome Research·M F BonaldoMarcelo B Soares
Aug 27, 1998·Insect Biochemistry and Molecular Biology·E M DotsonFrank H Collins
Nov 14, 1998·Seminars in Cell & Developmental Biology·M T Fuller
Apr 10, 1999·Insect Biochemistry and Molecular Biology·R L TellamP Willadsen
Sep 6, 2002·Insect Biochemistry and Molecular Biology·Coralia LunaLiangbiao Zheng
Oct 5, 2002·Science·Robert A HoltStephen L Hoffman
Jul 29, 2006·Malaria Journal·Bernadette J HuhoHeather M Ferguson
Jan 31, 2007·BMC Genomics·Emma WarrGeorge Dimopoulos
Jun 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Anastasios C KoutsosGeorge K Christophides
Apr 28, 2009·Gene·Chansak Suwanchaichinda, Michael R Kanost
Mar 17, 2010·BMC Genomics·Jiubo LiangNingjia He

Related Concepts

Metabolic Process, Cellular
Genes, Insect
Real-Time Polymerase Chain Reaction
Angle Closure Glaucoma
Molting
Immune Response
Body Part
Microarray Analysis
Biological Adaptation to Stress
Benign Neoplasm of Testis

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