Transcription profiling in environmental diagnostics: health assessments in Columbia River basin steelhead (Oncorhynchus mykiss)

Environmental Science & Technology
Richard E ConnonInge Werner

Abstract

The health condition of out-migrating juvenile salmonids can influence migration success. Physical damage, pathogenic infection, contaminant exposure, and immune system status can affect survival probability. The present study is part of a wider investigation of out-migration success in juvenile steelhead (Oncorhynchus mykiss) and focuses on the application of molecular profiling to assess sublethal effects of environmental stressors in field-collected fish. We used a suite of genes in O. mykiss to specifically assess responses that could be directly related to steelhead health condition during out-migration. These biomarkers were used on juvenile steelhead captured in the Snake River, a tributary of the Columbia River, in Washington, USA, and were applied on gill and anterior head kidney tissue to assess immune system responses, pathogen-defense (NRAMP, Mx, CXC), general stress (HSP70), metal-binding (metallothionein-A), and xenobiotic metabolism (Cyp1a1) utilizing quantitative polymerase chain reaction (PCR) technology. Upon capture, fish were ranked according to visual external physical conditions into good, fair, poor, and bad categories; gills and kidney tissues were then dissected and preserved for gene analyses. Transcri...Continue Reading

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Citations

Jul 7, 2016·Conservation Physiology·Christine L MadligerOliver P Love
Feb 28, 2014·Integrated Environmental Assessment and Management·Sharon E HookGraeme E Batley
Mar 27, 2015·Environmental Toxicology and Chemistry·Adam D BialesRichard E Connon
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Apr 21, 2017·Critical Reviews in Toxicology·Kristina RehbergerLisa Baumann
Jan 31, 2018·The Journal of Experimental Biology·Richard E ConnonNann A Fangue

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