Transcription profiling reveals stage- and function-dependent expression patterns in the filarial nematode Brugia malayi.

BMC Genomics
Ben-Wen LiGary J Weil

Abstract

Brugia malayi is a nematode parasite that causes lymphatic filariasis, a disfiguring and disabiling tropical disease. Although a first draft genome sequence was released in 2007, very little is understood about transcription programs that govern developmental changes required for the parasite's development and survival in its mammalian and insect hosts. We used a microarray with probes that represent some 85% of predicted genes to generate gene expression profiles for seven parasite life cycle stages/sexes. Approximately 41% of transcripts with detectable expression signals were differentially expressed across lifecycle stages. Twenty-six percent of transcripts were exclusively expressed in a single parasite stage, and 27% were expressed in all stages studied. K-means clustering of differentially expressed transcripts revealed five major transcription patterns that were associated with parasite lifecycle stages or gender. Examination of known stage-associated transcripts validated these data sets and suggested that newly identified stage or gender-associated transcripts may exercise biological functions in development and reproduction. The results also indicate that genes with similar transcription patterns were often involved ...Continue Reading

References

Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J A FuhrmanF B Perler
Oct 1, 1985·Molecular and Biochemical Parasitology·J A Fuhrman, W F Piessens
Jun 1, 1995·Experimental Parasitology·J A FuhrmanD Dalamagas
Nov 1, 1993·Bio/technology·D P McDonnellM A Gleeson
Jul 1, 1997·Molecular and Biochemical Parasitology·W F GregoryR M Maizels
May 30, 1998·The Journal of Biological Chemistry·K A RuudK S Browning
Dec 11, 1999·Molecular and Biochemical Parasitology·N RaghavanA L Scott
Oct 13, 2000·Molecular Cell·V ReinkeS K Kim
Nov 10, 2001·Annual Review of Phytopathology·D M BirdD L Baillie
Nov 15, 2001·The Journal of Biological Chemistry·Sarwar HashmiSara Lustigman
Jun 22, 2002·Molecular and Biochemical Parasitology·Collette Britton, Linda Murray
Sep 28, 2002·Science·Michelle N ArbeitmanKevin P White
Oct 24, 2002·International Journal for Parasitology·M CasiraghiC Bandi
Mar 7, 2003·Development·John Wang, Stuart K Kim
Mar 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michael P WashburnJohn R Yates
Sep 4, 2003·Genome Biology·Dov GreenbaumMark Gerstein
Oct 11, 2003·Trends in Genetics : TIG·Hui GeMarc Vidal
Mar 19, 2004·Current Biology : CB·Mitchell P Levesque, Philip N Benfey
Nov 1, 1996·Parasitology Today·E DevaneyF J Thompson
Sep 14, 2004·Immunological Reviews·Rick M MaizelsJudith E Allen
Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
Jun 28, 2005·Molecular and Biochemical Parasitology·Jennifer M MoserGreg Gibson
Aug 27, 2005·PLoS Biology·Alison R FrandGary Ruvkun
Jun 9, 2006·International Journal for Parasitology·S Nikolaou, R B Gasser
Dec 26, 2006·Cellular and Molecular Life Sciences : CMLS·H Nakamoto, L Vígh
Sep 22, 2007·Science·Elodie GhedinAlan L Scott
Nov 15, 2007·PloS One·Sanjay KumarClotilde K S Carlow
Nov 16, 2007·International Journal for Parasitology·Daojun JiangGary J Weil
Dec 1, 2007·WormBook : the Online Review of C. Elegans Biology·E Jane Albert Hubbard, David Greenstein
Jan 10, 2008·Trends in Parasitology·Alasdair J NisbetRobin B Gasser
Apr 9, 2008·Molecular and Biochemical Parasitology·Munirathinam GnanasekarKalyanasundaram Ramaswamy
Apr 29, 2008·Molecular and Biochemical Parasitology·James P HewitsonRick M Maizels
Oct 30, 2008·PLoS Neglected Tropical Diseases·Yovany Moreno, Timothy G Geary
Apr 9, 2009·PLoS Neglected Tropical Diseases·Sasisekhar BennuruThomas B Nutman

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Citations

Apr 11, 2013·Molecular Genetics and Genomics : MGG·Zhengyuan WangMakedonka Mitreva
May 24, 2014·PLoS Neglected Tropical Diseases·Young-Jun ChoiBruce M Christensen
Mar 6, 2013·PLoS Pathogens·Christina M TaylorMakedonka Mitreva
Jul 23, 2015·International Journal for Parasitology. Drugs and Drug Resistance·Ben-Wen LiGary J Weil
May 17, 2015·Trends in Parasitology·Andy AlhassanClotilde K S Carlow
Dec 4, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Samantha N McNultyPeter U Fischer
Apr 1, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Moorthy SakthideviPerumal Kaliraj
Sep 15, 2015·PLoS Neglected Tropical Diseases·C Paul MorrisEdward Mitre
May 23, 2015·PLoS Neglected Tropical Diseases·Rahul TyagiMakedonka Mitreva
Apr 21, 2017·PLoS Neglected Tropical Diseases·Sara LustigmanElodie Ghedin
Jun 6, 2019·Parasite : Journal De La Société Française De Parasitologie·Supaporn NuamtanongPoom Adisakwattana
Jun 24, 2020·PLoS Neglected Tropical Diseases·Alexandra GroteElodie Ghedin
May 10, 2019·Science Translational Medicine·Malina A BakowskiCase W McNamara
Oct 17, 2017·Trends in Parasitology·Sasisekhar BennuruThomas B Nutman

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Datasets Mentioned

BETA
AA570858

Methods Mentioned

BETA
protein folding

Software Mentioned

LIMMA
Clara
Nematode
InterProScan
Bioconductor

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