Transcription regulation of ompF and ompC by a single transcription factor, OmpR.

The Journal of Biological Chemistry
Takeshi YoshidaMasayori Inouye

Abstract

The ompF and ompC genes of Escherichia coli are reciprocally regulated by a single transcription factor, phosphorylated OmpR (OmpR-P), depending upon medium osmolarity. This regulation involves activation of ompF and its repression with concomitant activation of ompC. This occurs through OmpR-P binding to four (F1, F2, F3, and F4) and three (C1, C2, and C3) sites located upstream of the ompF and ompC promoters, respectively, through a novel mechanism. Here we show that there is a distinct OmpR-P binding hierarchy within F1, F2, and F3 sites as well as within C1, C2, and C3 sites. Each of these sites contains two tandem 10-bp OmpR-P-binding subsites, a-site and b-site (from 5' to 3' direction). OmpR-P has higher affinity to the downstream b-site than to the upstream a-site in each case. Six OmpR-P molecules bind to F and C sites two-by-two in a discontinuous "galloping" manner. We propose that this tight hierarchical binding of a transcription factor, OmpR, allows distinct stepwise regulation of ompF and ompC transcription, which minimizes their overlapping expression upon changes in the medium osmolarity to achieve the reciprocal expression of ompF and ompC.

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Citations

Sep 8, 2010·Nature Immunology·Scott B BergerCox Terhorst
Aug 20, 2009·The Journal of Biological Chemistry·Yoshihiro YamaguchiMasayori Inouye
Jan 16, 2008·The Journal of Biological Chemistry·Jee Eun RheeLinda J Kenney
Jun 27, 2008·The Journal of Biological Chemistry·Min Seong KwonH Moo Kwon
Jul 20, 2012·Molecular Plant-microbe Interactions : MPMI·David Zamorano-SánchezLourdes Girard
Apr 10, 2007·Journal of Bacteriology·Karl M ThompsonSusan Gottesman
Dec 11, 2007·Journal of Bacteriology·Akesh SinhaDibyendu Sarkar
Jun 2, 2011·Canadian Journal of Microbiology·He GaoRuifu Yang
Oct 8, 2013·Journal of Bacteriology·Yuta OkkotsuMichael J Schurr
Aug 30, 2008·Expert Opinion on Drug Delivery·Sampath C Abeylath, Edward Turos
Sep 4, 2015·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Tomohiro ShimadaAkira Ishihama
Feb 3, 2012·Molecular Microbiology·Yunzhou WeiBonnie L Bassler

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