Transcriptional activation of a moderately expressed tRNA gene by a positioned nucleosome

The Biochemical Journal
Akhila Parthasarthy, Karumathil P Gopinathan

Abstract

All of the members of a tRNA1(Gly) multigene family from the mulberry silkworm, Bombyx mori, have identical coding regions and consequently identical internal promoter elements, but are transcribed at different levels. A moderately expressed copy, tRNA1(Gly)-4 from within this multigene family, which was transcribed to 30-50% of the highly transcribed gene copies harboured two typical TATAA box sequences in the 5' upstream region at positions -27 nt and -154 nt with respect to the +1 nt of mature tRNA. Deletion of the distal TATAA sequence at -154 nt brought down the transcription more than 70%, whereas mutation of the proximal element did not affect transcription. tRNA1(Gly)-4 could be readily assembled into chromatin, with a positioned nucleosome in the upstream region, and the assembled nucleosome formed stable complexes with the transcription factors TFIIIC and TFIIIB. Organization of the gene into nucleosomes also enhanced transcription significantly above that of the naked DNA, reaching transcription levels comparable with those of the highly transcribed copies. This nucleosome-mediated enhancement in transcription was absent when the distal TATAA sequences were deleted, whereas mutation of the proximal TATAA element show...Continue Reading

References

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Jul 14, 2005·The Biochemical Journal·Akhila Parthasarthy, Karumathil P Gopinathan

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Citations

Feb 9, 2017·Scientific Reports·Alexandra Søgaard HelboKirsten Grønbæk
Jun 27, 2019·IUBMB Life·Guillaume HummelLaurence Drouard

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