Transcriptional Activation of Human GD3 Synthase (hST8Sia I) Gene in Curcumin-Induced Autophagy in A549 Human Lung Carcinoma Cells

International Journal of Molecular Sciences
Miri LeeYoung-Choon Lee

Abstract

Curcumin, a natural polyphenolic compound isolated from the plant Curcuma longa, is known to induce autophagy in various cancer cells, including lung cancer. In the present study, we also confirmed by LC3 immunofluorescence and immunoblotting analyses that curcumin triggers autophagy in the human lung adenocarcinoma A549 cell line. In parallel with autophagy induction, the gene expression of human GD3 synthase (hST8Sia I) responsible for ganglioside GD3 synthesis was markedly elevated in response to curcumin in the A549 cells. To investigate the transcriptional activation of hST8Sia I associated with the autophagy formation in curcumin-treated A549 cells, functional characterization of the 5'-flanking region of the hST8Sia I gene was carried out using the luciferase reporter assay system. Deletion analysis demonstrated that the -1146 to -646 region, which includes the putative c-Ets-1, CREB, AP-1, and NF-κB binding sites, functions as the curcumin-responsive promoter of hST8Sia I in A549 cells. The site-directed mutagenesis and chromatin immunoprecipitation assay demonstrated that the NF-κB binding site at -731 to -722 was indispensable for the curcumin-induced hST8Sia I gene expression in A549 cells. Moreover, the transcriptio...Continue Reading

References

Oct 20, 1995·The Journal of Biological Chemistry·S Singh, B B Aggarwal
Feb 1, 1997·Current Biology : CB·V R Baichwal, P A Baeuerle
Nov 16, 2002·International Immunopharmacology·Tieli WangJian Jian Li
Dec 17, 2002·Current Opinion in Hematology·Senitiroh Hakomori
Feb 20, 2004·Glycoconjugate Journal·Meryem Bektas, Sarah Spiegel
Jul 15, 2004·International Journal of Cancer. Journal International Du Cancer·Sita AggarwalBharat B Aggarwal
Jun 5, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yvonne G LinAnil K Sood
Jul 25, 2007·Current Problems in Cancer·Shishir ShishodiaBharat B Aggarwal
Sep 29, 2007·Biochemical Pharmacology·Ajay GoelBharat B Aggarwal
Jan 15, 2008·Cell·Beth Levine, Guido Kroemer
Jul 11, 2009·The AAPS Journal·Jayaraj RavindranBharat B Aggarwal
Jan 14, 2010·British Journal of Pharmacology·Jaegyu HwangKyoungho Suk
Mar 13, 2010·The Journal of Pathology·Danielle GlickKay F Macleod
Oct 6, 2010·Advances in Experimental Medicine and Biology·Sandro Sonnino, Alessandro Prinetti
Nov 13, 2010·Experimental & Molecular Medicine·Haw-Young KwonYoung-Choon Lee
Apr 5, 2011·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Youn Ju LeeChuhee Lee
May 5, 2012·Archives of Oral Biology·Ji Young KimHae Ryoun Park
May 23, 2012·Mechanisms of Ageing and Development·Grazyna MosieniakEwa Sikora
Feb 15, 2013·The New England Journal of Medicine·Augustine M K ChoiBeth Levine
Mar 28, 2013·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Bin LiNobuo Yaegashi
Sep 21, 2013·Journal of Pharmacological Sciences·Kui XiaoChunxue Bai
Oct 30, 2013·The FEBS Journal·Giovanni D'AngeloDomenico Russo
May 6, 2015·Journal of Hepatology·Jens U MarquardtSnorri S Thorgeirsson
Dec 27, 2016·Journal of Cellular Biochemistry·Hyunju ChoiCheorl-Ho Kim
Apr 4, 2017·International Journal of Biological Sciences·Kyung-Min KwonCheorl-Ho Kim
May 24, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Zahid HussainShahzeb Khan
Jul 5, 2017·Experimental and Therapeutic Medicine·Yaowu ZhangYi Lang
Jul 21, 2017·Journal of Traditional and Complementary Medicine·Matthew C FadusHenry T Lynch

❮ Previous
Next ❯

Citations

Jun 11, 2020·Journal of Cellular Physiology·Milad AshrafizadehSaeed Samarghandian
Sep 16, 2020·International Journal of Molecular Sciences·Monica BenvenutoRoberto Bei
Nov 22, 2019·Anti-cancer Agents in Medicinal Chemistry·Rumana AhmadArvind K Srivastava
Dec 12, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Miri LeeYoung-Choon Lee

❮ Previous
Next ❯

Datasets Mentioned

BETA
GMR19

Methods Mentioned

BETA
confocal microscopy
immunoprecipitation
PCR
ChIP
ELISA
Assay

Related Concepts

Related Feeds

Autophagy & Metabolism

Autophagy preserves the health of cells and tissues by replacing outdated and damaged cellular components with fresh ones. In starvation, it provides an internal source of nutrients for energy generation and, thus, survival. A powerful promoter of metabolic homeostasis at both the cellular and whole-animal level, autophagy prevents degenerative diseases. It does have a downside, however--cancer cells exploit it to survive in nutrient-poor tumors.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms