Transcriptional activation of NtcA-dependent promoters of Synechococcus sp. PCC 7942 by 2-oxoglutarate in vitro

Proceedings of the National Academy of Sciences of the United States of America
Ryohei TanigawaHideo Takahashi

Abstract

The transcription factor NtcA is a global regulator of nitrogen homeostasis in cyanobacteria. It thus positively regulates the expression of genes related to nitrogen assimilation such as glnA (which encodes glutamine synthetase) and ntcA itself in response to nitrogen shortage or depletion. The binding of NtcA to the glnA and ntcA promoters of Synechococcus sp. PCC 7942 in vitro now has been shown to be enhanced by 2-oxoglutarate. In vitro analysis of gene transcription also revealed that the interaction of NtcA with its promoter element was not sufficient for activation of transcription, and 2-oxoglutarate was required for transcriptional initiation by NtcA. Given that the intracellular concentration of 2-oxoglutarate is inversely related to nitrogen availability, it is proposed that this metabolite functions as a signaling molecule that transmits information on cellular nitrogen status to NtcA and thereby regulates the transcription of genes related to nitrogen assimilation in cyanobacteria.

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Citations

Sep 7, 2005·Photosynthesis Research·M Isabel Muro-PastorFrancisco J Florencio
Sep 7, 2005·Photosynthesis Research·Enrique FloresAntonia Herrero
Dec 10, 2003·Current Opinion in Microbiology·James W Golden, Ho-Sung Yoon
Dec 8, 2009·Nature Reviews. Microbiology·Enrique Flores, Antonia Herrero
Aug 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·José L LlácerVicente Rubio
Feb 2, 2011·Journal of Experimental Botany·Yoshitake OhashiTatsuo Omata
Jun 15, 2007·Plant & Cell Physiology·Takashi Osanai, Kan Tanaka
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Krithika KumarJames W Golden
Sep 3, 2004·The Plant Cell·George W Templeton, Greg B G Moorhead
Sep 6, 2006·Journal of Bacteriology·Elvira Olmedo-VerdAntonia Herrero
Jun 2, 2009·Journal of Bacteriology·Javier EspinosaAsunción Contreras
Nov 26, 2002·Journal of Bacteriology·Duan Liu, James W Golden
Oct 18, 2003·Journal of Bacteriology·Masahiko ImashimizuMikio Tsuzuki
Feb 3, 2006·Journal of Bacteriology·Samer SakrTania Arcondeguy
Jun 3, 2009·Microbiology and Molecular Biology Reviews : MMBR·D J ScanlanF Partensky
May 5, 2012·PloS One·Karim Boumediene LaichoubiAsunción Contreras
Mar 31, 2004·Proceedings of the National Academy of Sciences of the United States of America·Xu HuangJindong Zhao
Jul 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yunming ShiJindong Zhao
Jun 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sophie LaurentCheng-Cai Zhang

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