Transcriptional activation of Salmonella typhimurium invasion genes by a member of the phosphorylated response-regulator superfamily

Molecular Microbiology
C JohnstonS I Miller

Abstract

The Salmonella typhimurium PhoP-repressed locus prgHIJK encodes components of a sec-independent type III secretion apparatus. This apparatus is composed of at least 17 proteins encoded on a 40 kb pathogenicity Island located at centisome 63 on the S. typhimurium chromosome. The secretion apparatus and some of its targets, SapB, SapC and SspD, are necessary for epithelial cell invasion. The transcription of many invasion genes, including prgHIJK, is coordinately activated by HilA, a transcription factor encoded within the pathogenicity island. In this report we identify sirA, a gene located outside the pathogenicity island that is essential for induction of prgHIJK and hilA transcription. sirA encodes a 234-amino-acid protein that is essential for S. typhimurium Ssp (Salmonella secreted protein) secretion and invasion and is similar to response regulators of two-component regulatory systems. sirA-mutant phenotypes could be suppressed by two DNA clones from unlinked loci, designated sirB and sirC. These data suggest that SirA may be phosphorylated in response to S. typhimurium sensing a mammalian microenvironment. Furthermore, SirA phosphorylation is predicted to initiate a cascade of transcription-factor synthesis which results ...Continue Reading

Citations

Apr 6, 2004·Biotechnology Advances·David S Guttman
Aug 14, 2003·Trends in Microbiology·Peggy A Cotter, Allison M Jones
Apr 1, 1997·Trends in Microbiology·C A SchererS I Miller
Sep 19, 1997·Trends in Microbiology·E A Groisman, H Ochman
Jun 15, 1999·Trends in Microbiology·J L Rakeman, S I Miller
Jun 22, 2001·Microbes and Infection·I Hansen-Wester, M Hensel
Jan 5, 2002·Microbes and Infection·C P Lostroh, C A Lee
Dec 28, 1999·Microbes and Infection·N R Salama, S Falkow
Mar 6, 1999·Current Opinion in Microbiology·P A Cotter, J F Miller
Jun 11, 1998·Molecular Microbiology·R L Smith, M E Maguire
Feb 27, 1999·Molecular Microbiology·B M AhmerF Heffron
Apr 21, 1999·Molecular Microbiology·J DeiwickM Hensel
Jun 9, 2000·Molecular Microbiology·R L Lucas, C A Lee
Apr 23, 2002·Molecular Microbiology·Brian K HammerMichele S Swanson
Dec 3, 1999·Immunological Reviews·J C SirardJ P Kraehenbuhl
Aug 5, 2000·Applied and Environmental Microbiology·R H Ffrench-ConstantF R Blattner
Nov 18, 2000·Infection and Immunity·A M Prouty, J S Gunn
Nov 18, 2000·Infection and Immunity·C AltierS D Lawhon
Apr 22, 2008·Journal of Bacteriology·Yanyan HuangCraig Altier

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.