Transcriptional activation of the porcine P450 11A insulin-like growth factor response element in MCF-7 breast cancer cells.

The Journal of Biological Chemistry
Randall J Urban, Yvonne H Bodenburg

Abstract

Insulin-like growth factor-I (IGF-I) stimulates the growth of MCF-7 breast cancer cells. This study determined the transcriptional activity of an IGF-I-responsive region (IGFRE) of porcine P450 11A (P450scc) after transfection into MCF-7 cells. IGF-I induced transcriptional activity of a porcine P450scc core promoter luciferase construct containing the IGFRE transfected in MCF-7 cells. Electrophoretic mobility shift assay with nuclear protein extract from MCF-7 cells showed two transcription factors binding to the IGFRE. Supershift assay determined that one transcription factor was Sp1. Electrophoretic mobility shift assay and transfection experiments with selected mutations to the IGFRE found that binding of both transcription factors was necessary to confer an IGF-I response. The binding activity of both transcription factors was increased with IGF-I treatment. In conclusion, MCF-7 cells contain Sp1 and another unknown transcription factor, P2, that bind to a known IGFRE (porcine P450scc) and induce reporter gene transcriptional activity with IGF-I treatment. Because Sp1 is a ubiquitous transcription factor, determining the identity of P2 may lead to cell-specific methods to impair breast cancer cell growth as mediated by IGF-I.

References

Mar 1, 1992·British Journal of Cancer·V M Macaulay
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J M Berg
Mar 16, 1992·Biochemical and Biophysical Research Communications·J AlemanyF De Pablo
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J AlemanyF de Pablo
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P HenthornT Kadesch
Jan 1, 1995·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·E ToropainenK Syrjänen
Jan 1, 1995·Annals of Internal Medicine·D LeRoithC T Roberts
Feb 1, 1995·Endocrine Reviews·J I Jones, D R Clemmons
Nov 15, 1993·Biochemical and Biophysical Research Communications·C B RichJ A Foster

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Citations

Feb 2, 2000·Journal of Cellular Biochemistry·A StoicaM B Martin
Aug 24, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·S KondoL J Sandell
Dec 7, 2007·American Journal of Physiology. Endocrinology and Metabolism·Sekar NatesampillaiJohannes D Veldhuis
Jun 26, 2001·Journal of Cellular Physiology·A R BlackJ Azizkhan-Clifford
Aug 13, 2008·Molecular and Cellular Endocrinology·Venkataraman SriramanRandall J Urban
Feb 22, 2005·Archives of Oral Biology·Javier CatónMargarita Zeichner-David
Sep 10, 2002·American Journal of Physiology. Endocrinology and Metabolism·Randall J Urban, Yvonne Bodenburg
Aug 10, 2002·American Journal of Physiology. Endocrinology and Metabolism·Randall J UrbanThomas G Wood
Jan 30, 2004·American Journal of Physiology. Endocrinology and Metabolism·Randall J UrbanJorge Chedrese

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