Transcriptional activity of heat shock factor 1 at 37 degrees C is repressed through phosphorylation on two distinct serine residues by glycogen synthase kinase 3 and protein kinases Calpha and Czeta.

The Journal of Biological Chemistry
B ChuS K Calderwood

Abstract

Heat shock factor 1 (HSF1) is the key transcriptional regulator of the heat shock genes that protect cells from environmental stress. However, because heat shock gene expression is deleterious to growth and development, we have examined mechanisms for HSF1 repression at growth temperatures, focusing on the role of phosphorylation. Mitogen-activated protein kinases (MAPKs) of the ERK family phosphorylate HSF1 and represses transcriptional function. The mechanism of repression involves initial phosphorylation by MAP kinase on serine 307, which primes HSF1 for secondary phosphorylation by glycogen synthase kinase 3 on a key residue in repression (serine 303). In vivo expression of glycogen synthase kinase 3 alpha or beta thus represses HSF1 through phosphorylation of serine 303. HSF1 is also phosphorylated by MAPK in vitro on a second residue (serine 363) adjacent to activation domain 1, and this residue is additionally phosphorylated by protein kinase C. In vivo, HSF1 is repressed through phosphorylation of this residue by protein kinase Calpha or -zeta but not MAPK. Regulation at 37 degrees C, therefore, involves the action of three protein kinase cascades that repress HSF1 through phosphorylation of serine residues 303, 307, an...Continue Reading

References

Aug 1, 1992·Biochemical Society Transactions·A R OlivierP J Parker
Aug 1, 1992·Genes & Development·J H FederS Lindquist
Jan 1, 1990·Methods in Enzymology·P Matsudaira
Jan 1, 1991·Methods in Enzymology·B E Kemp, R B Pearson
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S K RabindranC Wu
Sep 30, 1991·Biochemical and Biophysical Research Communications·N MaruiM Fukushima
Jun 1, 1991·Molecular and Cellular Biology·B D Price, S K Calderwood
Jan 1, 1988·Annual Review of Genetics·S Lindquist, E A Craig
Sep 5, 1988·Journal of Molecular Biology·P SchillerR Voellmy
Feb 1, 1994·Current Opinion in Genetics & Development·C J Marshall
Jan 1, 1993·Annual Review of Cell Biology·C Georgopoulos, W J Welch
Mar 1, 1996·Molecular and Cellular Biology·E M NewtonR E Kingston
Jan 1, 1995·Annual Review of Cell and Developmental Biology·C Wu
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·H KonishiU Kikkawa
Mar 7, 1997·The Journal of Biological Chemistry·C I HolmbergL Sistonen
Feb 1, 1997·Protein Expression and Purification·F SoncinS K Calderwood
Mar 1, 1997·Cell Stress & Chaperones·R A Krebs, M E Feder
Oct 27, 1997·The Journal of Biological Chemistry·C ChenS K Calderwood

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Citations

Jul 20, 2012·Molecular and Cellular Biochemistry·Harlokesh Narayan YadavPyare Lal Sharma
Aug 23, 2008·Cell Stress & Chaperones·Philip L Hooper, Paul L Hooper
Aug 30, 2011·Cell Stress & Chaperones·Jordan Thomas Silver, Earl G Noble
Mar 28, 2003·Biochemical and Biophysical Research Communications·Fabrice SoncinStuart K Calderwood
Aug 31, 2001·Progress in Neurobiology·C A Grimes, R S Jope
Nov 24, 2001·Brain Research Bulletin·M RimoldiV Zimarino
Dec 7, 2002·Trends in Biochemical Sciences·Carina I HolmbergLea Sistonen
Dec 2, 2011·Nature Reviews. Drug Discovery·Daniel W NeefDennis J Thiele
Sep 21, 2001·The Biochemical Journal·S Frame, P Cohen
Jun 16, 2012·Molecular & Cellular Proteomics : MCP·Agata M Trzcinska-DanelutiDaniela Rotin
Mar 12, 2005·BMC Biochemistry·Toumy GuettoucheRichard Voellmy
Nov 19, 2010·PloS One·Ayesha MurshidStuart K Calderwood
Sep 27, 2000·Cell Stress & Chaperones·C I HolmbergL Sistonen
Feb 24, 2001·Cell Stress & Chaperones·X WangS K Calderwood
Apr 24, 2008·Molecular Medicine·James P Stice, Anne A Knowlton
Jun 2, 2011·International Journal of Alzheimer's Disease·Marjelo A MinesRichard S Jope
Feb 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·I Szanto, C R Kahn
Mar 23, 2011·Annual Review of Biochemistry·Julius Anckar, Lea Sistonen
Mar 14, 2014·Nutritional Neuroscience·Eka FujimotoKazuto Sato
Sep 16, 2006·Molecular Cell·Xiang-Jiao Yang, Serge Grégoire
Jun 24, 2004·Progress in Nucleic Acid Research and Molecular Biology·Richard Voellmy
Apr 28, 2009·Advanced Drug Delivery Reviews·Wolfgang Walther, Ulrike Stein
Feb 15, 2017·Nature Communications·Rocio Gomez-PastorDennis J Thiele
Apr 1, 2015·Molecular & Cellular Proteomics : MCP·Agata M Trzcińska-DanelutiDaniela Rotin
Mar 3, 2004·Genesis : the Journal of Genetics and Development·Guanghu WangNahid F Mivechi
Aug 31, 2017·Nature Reviews. Molecular Cell Biology·Rocio Gomez-PastorDennis J Thiele
Jan 1, 2010·Signal Transduction Insights·S K CalderwoodY Zhang
Jun 30, 2016·Molecular and Cellular Biology·Sharadha Dayalan NaiduAlbena T Dinkova-Kostova

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