Transcriptional activity of HPV in inverted papilloma demonstrated by in situ hybridization for E6/E7 mRNA

Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery
David G StoddardErin K O'Brien

Abstract

Assess human papilloma virus (HPV) transcriptional activity in inverted Schneiderian papillomas (IPs). Case series with chart review. Academic tertiary care center. Retrospective clinicopathologic review of 19 cases of IP in patients undergoing surgical excision from 1995 to 2013 at Mayo Clinic in Rochester, Minnesota. Surgical pathology archival material was histopathologically reviewed using hematoxylin and eosin-stained slides. Formalin-fixed, paraffin-embedded material from each case was evaluated for p16 expression using immunohistochemistry as well as HPV DNA and E6/E7 messenger RNA (mRNA) transcription using polymerase chain reaction (PCR) and in situ hybridization (via RNAscope technology), respectively. Eight patients were female (42%), with an average age of 53 years (range, 23-82 years). Three demonstrated malignancy, and 5 subsequently recurred. Average follow-up was 49 months (range, 0-200 months), and 1 patient died from squamous cell carcinoma arising from the IP. RNAscope detected HPV mRNA transcripts exclusively within IP in 100% of cases; however, in 11 patients (58%), less than 1% of cells exhibited transcriptional activity. Only 2 of 19 cases (11%) demonstrated mRNA activity in 50% or more cells. HPV DNA was...Continue Reading

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Oct 21, 2015·Seminars in Diagnostic Pathology·Selvam Thavaraj
Apr 19, 2018·The Laryngoscope·Sepideh MohajeriShaun Kilty
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Methods Mentioned

BETA
PCR
biopsy

Software Mentioned

RNAscope

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Carcinoma, Squamous Cell

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