Transcriptional activity of the DeltaNp63 promoter is regulated by STAT3

The Journal of Biological Chemistry
Wing-Keung ChuJan-Kan Chen

Abstract

The N terminus-truncated splicing variant of TAp63 is known as DeltaNp63. DeltaNp63 lacks transactivation function and is thought to antagonize the transcriptional regulation of the p53 and TAp63 target genes. Overexpression of DeltaNp63 has been observed in a number of human cancers, suggesting a role in carcinogenesis. In the present study we present data showing that the DeltaNp63 gene promoter activity is positively regulated by DeltaNp63alpha, and such positive autoregulation is mediated via activation of STAT3 activity. We show that expression of DeltaNp63alpha in Hep3B cells induces Stat3 phosphorylation on Tyr-705 and Ser-727. A putative STAT3-responsive element (STAT3-RE) is identified in the DeltaNp63 promoter region. Electrophoretic mobility shift and avidin biotin-Conjugated DNA assays show direct binding of STAT3 to STAT3-RE of the DeltaNp63 promoter, and such binding is stimulated by DeltaNp63alpha. Binding of the endogenous STAT3 to the DeltaNp63 promoter in Hep3B cells was demonstrated by a chromatin immunoprecipitation assay. The stimulation of the DeltaNp63 transcriptional activity by DeltaNp63alpha is abolished by Janus kinase 2 (JAK2)/STAT3 inhibitor AG490, dominant-negative STAT3, STAT3 small interfering RN...Continue Reading

References

Mar 15, 1996·The Journal of Biological Chemistry·A KapteinM Saunders
Aug 24, 1999·Cell·J F BrombergJ E Darnell
Apr 13, 2000·Japanese Journal of Cancer Research : Gann·S IshidaT Tokino
Jun 13, 2000·Oncogene·T BowmanR Jove
Jun 13, 2000·Oncogene·T Decker, P Kovarik
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·G PellegriniM De Luca
Feb 5, 2003·Biochemical and Biophysical Research Communications·Biao HeDavid M Jablons
May 30, 2003·The Biochemical Journal·Peter C HeinrichFred Schaper
Oct 14, 2003·The Journal of Biological Chemistry·Christopher E BarbieriJennifer A Pietenpol
Oct 28, 2003·Investigative Ophthalmology & Visual Science·Der-Yuan WangJan-Kan Chen
Mar 23, 2004·Carcinogenesis·Matthew D Westfall, Jennifer A Pietenpol
Mar 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hans-Ingo TrompeterMarkus Uhrberg
Mar 7, 2006·Biochemical and Biophysical Research Communications·Wing-Keung ChuJan-Kan Chen
Apr 18, 2006·Biochemical and Biophysical Research Communications·Hae-ock LeeHyunsook Lee

❮ Previous
Next ❯

Citations

Apr 3, 2014·Endocrine-related Cancer·Shahin AssefniaPriscilla A Furth
Jun 24, 2017·Proceedings of the National Academy of Sciences of the United States of America·Daniel B HerrickJames E Schwob
Mar 29, 2018·Cellular & Molecular Biology Letters·Michaela GaloczovaBorivoj Vojtesek
Nov 2, 2017·Bioscience, Biotechnology, and Biochemistry·Jeng-Yuan Yao, Jan-Kan Chen
Aug 24, 2018·Genes & Development·Stephen D'AmicoNancy C Reich
Aug 23, 2020·International Journal of Oral Science·Mohit SharmaRaghu Radhakrishnan
Jul 19, 2012·Journal of Cellular Physiology·Yi-Jen HsuehJan-Kan Chen
Aug 14, 2020·International Journal of Molecular Sciences·Martina MinoliRoland Seiler
Jul 8, 2010·Journal of Cellular Physiology·Jeng-Yuan YaoJan-Kan Chen
Apr 22, 2015·Biochemical and Biophysical Research Communications·Masaya KatoTadashi Matsuda
Feb 28, 2021·The Journal of Pathology·Zuzana PokornáPhilip J Coates
Oct 7, 2008·Mechanisms of Development·Nadia DanilovaShuo Lin

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.