Transcriptional analysis of brown adipose tissue in leptin-deficient mice lacking inducible nitric oxide synthase: evidence of the role of Med1 in energy balance

Physiological Genomics
Sara BecerrilGema Frühbeck

Abstract

Leptin and nitric oxide (NO) are implicated in the control of energy homeostasis. The aim of the present study was to examine the impact of the absence of the inducible NO synthase (iNOS) gene on the regulation of energy balance in ob/ob mice analyzing the changes in gene expression levels in brown adipose tissue (BAT). Double knockout (DBKO) mice simultaneously lacking the ob and iNOS genes were generated and the expression of genes involved in energy balance including fatty acid and glucose metabolism as well as mitochondrial genes were analyzed by microarrays. DBKO mice exhibited an improvement in energy balance with a decrease in body weight (P < 0.001), total fat pads (P < 0.05), and food intake (P < 0.05), as well as an enhancement in BAT function compared with ob/ob mice. To better understand the molecular events associated with this improvement, BAT gene expression was analyzed. Of particular interest, gene expression levels of the key subunit of the Mediator complex Med1 was upregulated (P < 0.05) in DBKO mice. Real-time PCR and immunohistochemistry further confirmed this data. Med1 is implicated in adipogenesis, lipid metabolic and biosynthetic processes, glucose metabolism, and mitochondrial metabolic pathways. Med1 ...Continue Reading

References

Jul 28, 1995·Science·M A PelleymounterF Collins
Mar 1, 1995·Analytical Biochemistry·G FrühbeckS Santidrián
Jul 1, 1997·The American Journal of Physiology·P J ScarpaceN Tümer
Nov 20, 1997·The Journal of Clinical Investigation·C Nathan
Dec 17, 1997·Biochemical and Biophysical Research Communications·G FrühbeckJ A Martínez
Sep 15, 1998·Biochemical and Biophysical Research Communications·G FrühbeckJ A Martínez
Feb 9, 2000·Diabetologia·G Frühbeck, J Salvador
Sep 5, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G Frühbeck, J Gómez-Ambrosi
Oct 5, 2001·Cellular Signalling·G Frühbeck, J Gómez-Ambrosi
Aug 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·James M NtambiAlan D Attie
Mar 11, 2003·Diabetologia·G Frühbeck, J Gómez-Ambrosi
Jun 20, 2003·Cell Death and Differentiation·E Bossy-Wetzel, S A Lipton
Nov 25, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Javier Gómez-AmbrosiGema Frühbeck
Jan 13, 2004·Physiological Reviews·Barbara Cannon, Jan Nedergaard
Apr 28, 2004·Diabetes·Alex W CohenMichael P Lisanti
Sep 7, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Aparna VenkatramanVictor M Darley-Usmar
Nov 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Enzo NisoliEmilio Clementi
Jan 22, 2005·Science·Bradford B Lowell, Gerald I Shulman
Feb 25, 2005·Lipids·Subrahmanyam S Chirala, Salih J Wakil
Jan 7, 2006·Nature Medicine·Johan Auwerx
Apr 25, 2006·Reviews of Physiology, Biochemistry and Pharmacology·M Belakavadi, J D Fondell
Sep 19, 2006·The Journal of Clinical Investigation·Alessandra ValerioEnzo Nisoli
Apr 17, 2007·Trends in Endocrinology and Metabolism : TEM·Gema FrühbeckCarlos Diéguez
May 4, 2007·Trends in Cell Biology·Jérôme N Feige, Johan Auwerx
Jul 21, 2007·Endocrinology·Kiymet BozaogluDavid Segal
Nov 8, 2007·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Kerstin NauMartin Klingenspor
May 17, 2008·Genes & Development·Shingo KajimuraBruce M Spiegelman
Mar 17, 2009·Trends in Cell Biology·Gema FrühbeckMaría A Burrell
May 26, 2009·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·V CatalánG Frühbeck

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Citations

May 30, 2014·Nutrition Research Reviews·Gema FrühbeckAmaia Rodríguez
Mar 13, 2014·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Zeliha Kapusuz GencerLevent Saydam
Apr 23, 2014·Biochimica Et Biophysica Acta·Concetta SchianoClaudio Napoli
Dec 26, 2013·Mitochondrion·Jialing YuPingping Jiang
Sep 10, 2014·International Journal of Obesity : Journal of the International Association for the Study of Obesity·A RodríguezG Frühbeck
Apr 5, 2016·Acta Physiologica·A RodríguezG Frühbeck
Feb 17, 2018·International Journal of Obesity : Journal of the International Association for the Study of Obesity·S BecerrilG Frühbeck
Sep 4, 2015·American Journal of Physiology. Endocrinology and Metabolism·Amaia RodriguezGema Frühbeck
Apr 3, 2020·Journal of Physiology and Biochemistry·A RodríguezSara Becerril
Sep 11, 2019·Nutrients·Sara BecerrilGema Frühbeck
May 7, 2020·Protein and Peptide Letters·Sara ParrettiniGiovanni Luca

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