Transcriptional analysis of differential carbohydrate utilization by Clostridium acetobutylicum

Microbiology
Matthew D ServinskyChristian J Sund

Abstract

Transcriptional analysis was performed on Clostridium acetobutylicum with the goal of identifying sugar-specific mechanisms for the transcriptional regulation of transport and metabolism genes. DNA microarrays were used to determine transcript levels from total RNA isolated from cells grown on media containing eleven different carbohydrates, including two pentoses (xylose, arabinose), four hexoses (glucose, mannose, galactose, fructose), four disaccharides (sucrose, lactose, maltose, cellobiose) and one polysaccharide (starch). Sugar-specific induction of many transport and metabolism genes indicates that these processes are regulated at the transcriptional level and are subject to carbon catabolite repression. The results show that C. acetobutylicum utilizes symporters and ATP-binding cassette (ABC) transporters for the uptake of pentose sugars, while disaccharides and hexoses are primarily taken up by phosphotransferase system (PTS) transporters and a gluconate : H(+) (GntP) transporter. The transcription of some transporter genes was induced by specific sugars, while others were induced by a subset of the sugars tested. Sugar-specific transport roles are suggested, based on expression comparisons, for various transporters of...Continue Reading

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Citations

May 12, 2012·Applied Microbiology and Biotechnology·Dörte LehmannTina Lütke-Eversloh
Aug 28, 2012·Journal of Industrial Microbiology & Biotechnology·M D ServinskyC J Sund
May 10, 2013·Biotechnology for Biofuels·Chenggang XuJian Xu
May 21, 2014·Applied Microbiology and Biotechnology·Christine VoigtRalf-Jörg Fischer
Sep 19, 2014·Microbial Cell Factories·Matthew D ServinskyChristian J Sund
Jan 11, 2016·World Journal of Microbiology & Biotechnology·Wilfrid J Mitchell
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Dec 8, 2014·Microbiology·Christian J SundMargaret M Hurley
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Apr 19, 2015·Microbial Cell Factories·Manuel BecerraMaría Isabel González-Siso
May 28, 2016·Environmental Technology·Rosemeri I DamsSandra T Santaella

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