Transcriptional analysis of novel hormone receptors PGRMC1 and PGRMC2 as potential biomarkers of breast adenocarcinoma staging.

The Journal of Surgical Research
Marlin Wayne CauseyJonathan D Stallings

Abstract

The expression of progesterone receptor membrane component 1 (PGRMC1) in breast cancer has generated interest in this recently discovered protein because of its role in tumorigenesis. However, correlations between patient age, PGRMC1 gene expression, breast cancer morphology, and breast cancer stage have not been adequately studied. Furthermore, very little is known about possible roles for other PGRMC isoforms in breast cancer, like PGRMC2. Thus, we examined the expression of PGRMC1 and PGRMC2 mRNA by relative quantitative PCR (RelqPCR) and determined whether transcript levels correlate with age, breast cancer staging, estrogen receptor alpha (ERα) status, and other morphometric features routinely used during the pathological examination of breast ductal adenocarcinomas. Twenty-eight frozen or paraffin embedded breast cancer samples (ductal carcinoma in situ and stages I thru IV invasive ductal adenocarcinoma) and 10 control benign breast tissue samples were randomly selected and interrogated by RelqPCR to determine PGRMC1, 2, and ERα mRNA transcript levels. To control for slight variations in sample preparation, receptor transcript was normalized to the housekeeping gene phosphoglycerate kinase 1 (PGK1). Descriptive statistic...Continue Reading

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Citations

Sep 3, 2013·The Journal of Steroid Biochemistry and Molecular Biology·A O MueckH Neubauer
Jun 14, 2013·Climacteric : the Journal of the International Menopause Society·H NeubauerA O Mueck
Jan 11, 2014·IEEE/ACM Transactions on Computational Biology and Bioinformatics·Chanchala D KaddiMay D Wang
Sep 27, 2012·Fertility and Sterility·Bruce PierRichard O Burney
Nov 6, 2018·BMC Bioinformatics·Xin-Ping XieHong-Qiang Wang
May 1, 2019·Scientific Reports·Diane OjoDamu Tang
Oct 16, 2018·Frontiers in Endocrinology·Xin-Jun WuYong Zhu

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