Transcriptional Analysis of Resistance to Low Temperatures in Bermudagrass Crown Tissues

PloS One
Kalpalatha MelmaieePatricia Canaan

Abstract

Bermudagrass (Cynodon dactylon L pers.) is one of the most geographically adapted and utilized of the warm-season grasses. However, bermudagrass adaptation to the Northern USA is limited by freeze damage and winterkill. Our study provides the first large-scale analyses of gene expression in bermudagrass regenerative crown tissues during cold acclimation. We compared gene expression patterns in crown tissues from highly cold tolerant "MSU" and susceptible "Zebra" genotypes exposed to near-freezing temperatures. Suppressive subtractive hybridization was used to isolate putative cold responsive genes Approximately, 3845 transcript sequences enriched for cold acclimation were deposited in the GenBank. A total of 4589 ESTs (3184 unigenes) including 744 ESTs associated with the bermudagrass disease spring dead spot were printed on microarrays and hybridized with cold acclimated complementary Deoxyribonucleic acid (cDNA). A total of 587 differentially expressed unigenes were identified in this study. Of these only 97 (17%) showed significant NCBI matches. The overall expression pattern revealed 40% more down- than up-regulated genes, which was particularly enhanced in MSU compared to Zebra. Among the up-regulated genes 68% were unique...Continue Reading

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Datasets Mentioned

BETA
DN989227.1
DN985375.1

Methods Mentioned

BETA
PCR
electrophoresis
transgenic
Reverse Transcription PCR

Software Mentioned

Bioconductor
- JMP - Pro
Pipeonline
Venny
SAS
BLASTx
Analysis of Variance (
CAP3
Excel
LIMMA

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