Transcriptional Analysis of T Cells Resident in Human Skin

PloS One
Jane LiJoel Z Ma

Abstract

Human skin contains various populations of memory T cells in permanent residence and in transit. Arguably, the best characterized of the skin subsets are the CD8(+) permanently resident memory T cells (TRM) expressing the integrin subunit, CD103. In order to investigate the remaining skin T cells, we isolated skin-tropic (CLA(+)) helper T cells, regulatory T cells, and CD8(+) CD103(-) T cells from skin and blood for RNA microarray analysis to compare the transcriptional profiles of these groups. We found that despite their common tropism, the T cells isolated from skin were transcriptionally distinct from blood-derived CLA(+) T cells. A shared pool of genes contributed to the skin/blood discrepancy, with substantial overlap in differentially expressed genes between each T cell subset. Gene set enrichment analysis further showed that the differential gene profiles of each human skin T cell subset were significantly enriched for previously identified TRM core signature genes. Our results support the hypothesis that human skin may contain additional TRM or TRM-like populations.

References

Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Mar 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rachael A ClarkThomas S Kupper
Aug 26, 2006·Nature Reviews. Immunology·William W Agace
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Oct 17, 2009·The Journal of Antibiotics·Takao Kataoka
Nov 20, 2009·Nucleic Acids Research·Nuno L Barbosa-MoraisSimon Tavaré
Oct 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Linda M WakimMichael J Bevan
Mar 17, 2011·Nature Reviews. Microbiology·Elizabeth A Grice, Julia A Segre
Jul 9, 2011·Nature Immunology·Brian S Sheridan, Leo Lefrançois
Nov 19, 2011·Nucleic Acids Research·Thomas KelderAlexander R Pico
Nov 22, 2011·Biostatistics·Johann A Gagnon-Bartsch, Terence P Speed
Aug 28, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Linda M WakimMichael J Bevan
Oct 6, 2012·Trends in Immunology·Thomas GebhardtFrancis R Carbone
Dec 21, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shannon K BromleyAndrew D Luster
Feb 7, 2013·Nature Communications·Teruaki NakatsujiRichard L Gallo
Apr 2, 2013·Nature Immunology·Jason M SchenkelDavid Masopust
Oct 29, 2013·Nature Immunology·Laura K MackayThomas Gebhardt
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ali ZaidScott N Mueller
Jul 30, 2014·Frontiers in Immunology·Damian Lanz Turner, Donna L Farber
Jan 22, 2015·Nucleic Acids Research·Matthew E RitchieGordon K Smyth
Feb 24, 2015·PLoS Computational Biology·Martina KutmonChris T Evelo
May 11, 2015·Cell·Elizabeth M SteinertDavid Masopust
Jun 30, 2015·Nature Medicine·Chang Ook Park, Thomas S Kupper

❮ Previous
Next ❯

Citations

Sep 13, 2016·The Journal of Clinical Investigation·Chandra Sekhar BoddupalliMadhav V Dhodapkar
Oct 28, 2017·Journal of Translational Medicine·Jeong-Hee Yang, Seok-Chan Eun
Feb 7, 2019·Annual Review of Immunology·David Masopust, Andrew G Soerens
Sep 2, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mariana SilvaRobert Sackstein
Mar 8, 2019·Cellular & Molecular Immunology·Jasper IskeStefan G Tullius
Sep 20, 2020·Science Immunology·Jenna L PappalardoDavid A Hafler
Nov 17, 2016·Nature Immunology·Sathi Wijeyesinghe, David Masopust
Jun 22, 2018·Frontiers in Immunology·Michelle S CruzJulie Marie Jameson
Aug 16, 2018·Frontiers in Immunology·Michel EnamoradoDavid Sancho
Jan 23, 2021·Science·Gary ReynoldsMuzlifah Haniffa
Mar 10, 2021·Cold Spring Harbor Perspectives in Biology·Feline E DijkgraafTon N M Schumacher

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE74158

Methods Mentioned

BETA
FCS
Flow Cytometry
fluorescence activated cell sorting
chips
PCA
PCR
transgenic

Software Mentioned

PathVisio
PANTHER
ruv R package
R limma package
limma
GO
. db
XLSTAT
Bioconductor
GSEA

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

The Journal of Immunology : Official Journal of the American Association of Immunologists
Linda M WakimMichael J Bevan
The Journal of Immunology : Official Journal of the American Association of Immunologists
Patrick SchaerliBernhard Moser
© 2021 Meta ULC. All rights reserved