PMID: 7539417Jun 1, 1995Paper

Transcriptional analysis of the amidase operon from Pseudomonas aeruginosa

Journal of Bacteriology
S A Wilson, R E Drew

Abstract

The transcriptional start point for the amidase structural gene (amiE) of Pseudomonas aeruginosa has been identified, and the promoter (pE) has been shown to function constitutively, as predicted for a system regulated by transcription antitermination. Northern (RNA) analysis results show that in cells grown under inducing conditions, a major 1.3-kb amiE transcript arises from pE, and in addition, a larger transcript of approximately 5.0 kb in length has been shown to derive from the same promoter, encoding all of the genes of the operon. DNA sequencing and S1 nuclease mapping have located a transcription terminator downstream of amiE, which terminates approximately half of the pE transcripts. Previously, two RpoN-dependent promoter-like sequences (pN1 and pN2) were identified upstream of the negative regulator gene, amiC, and we have now constructed a promoter probe vector which shows weak constitutive promoter activity within this region. This promoter would be expected to provide basal levels of expression of the amiC and amiR regulatory genes to allow induction of the system.

References

Aug 1, 1978·Journal of Bacteriology·F Farin, P H Clarke
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
May 11, 1987·FEBS Letters·W J BrammarP H Clarke
Dec 1, 1964·Journal of General Microbiology·W J BRAMMAR, P H CLARKE
Feb 1, 1962·Journal of General Microbiology·M KELLY, P H CLARKE

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Citations

Jan 1, 1996·Annual Review of Genetics·T M Henkin
Jul 13, 2000·The Journal of Biological Chemistry·R A NormanR E Drew
Jan 25, 2017·Scientific Reports·Thomas ClamensOlivier Lesouhaitier
Sep 26, 2014·Environmental Science and Pollution Research International·A G GuezennecM Motelica-Heino
Jan 27, 1999·Biochemical and Biophysical Research Communications·M KobayashiS Shimizu

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