Transcriptional analysis of the candidate spermatogenesis gene Ube1y and of the closely related Ube1x shows that they are coexpressed in spermatogonia and spermatids but are repressed in pachytene spermatocytes

Developmental Biology
T OdorisioP S Burgoyne

Abstract

Ube1y is a Y-linked gene transcribed in the testis, which maps to a region of the mouse Y required for normal spermatogonial proliferation. Ube1y, together with a ubiquitously expressed homologue on the X chromosome (Ube1x), encodes ubiquitin-activating enzyme E1, an enzyme essential for eukaryotic cell proliferation. Ube1y is thus a strong candidate for the Y function in spermatogonial proliferation. Using probes specific for the two genes, we have used Northern analysis and RNase protection to assess transcript levels throughout testis development and, by using germ cell-deficient XXSxr(a) testes and purified cell fractions, we have defined the testicular cell types in which transcription occurs. Ube1y transcripts are already detectable in the fetal testis at 12.5 dpc, with higher levels at 14.5 dpc and then falling to low levels by the time of birth. Postnatally levels rise sharply, peaking at 10 dpp. Analysis of XXSxr(a) testes indicates that the bulk of the Ube1y transcription is in germ cells. The analysis of purified cell fractions shows that X- and Y-encoded transcripts are present in A spermatogonia, both are at very low levels (or perhaps absent) in pachytene spermatocytes and then return to high levels in round sperm...Continue Reading

Citations

Sep 13, 2000·Molecular Reproduction and Development·K MochidaA L Kierszenbaum
Sep 23, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D J Elliott, H J Cooke
Mar 29, 2000·Theriogenology·P J Hendriksen
Jan 28, 1999·Molecular and Cellular Endocrinology·J A GrootegoedJ H Hoeijmakers
Jul 21, 2009·Molecular & Cellular Proteomics : MCP·Katherine EwenPeter Koopman
Feb 5, 2008·Yi chuan = Hereditas·Shao-Qin GeShu-Mei Mu
Feb 2, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Michelle AltonTeruko Taketo
Apr 13, 2005·Human Fertility : Journal of the British Fertility Society·Darren K Griffin, Katie A Finch
Jun 28, 2016·Molecular Cytogenetics·Galina N PochukalinaAlexey P Voronin
Jul 29, 2006·Gene Expression Patterns : GEP·Fang YangP Jeremy Wang
May 20, 2003·Molecular & Cellular Proteomics : MCP·Emmanuelle ComCharles Pineau
May 28, 2004·Human Molecular Genetics·Julian P VenablesDavid J Elliott
Dec 13, 2005·Nature Genetics·Di Kim Nguyen, Christine M Disteche
Jun 5, 2003·Molecular Reproduction and Development·Denise EscalierXin Xu
Nov 16, 2002·Genesis : the Journal of Genetics and Development·John R McCarreyStephen A Krawetz
Mar 21, 2001·Nature Reviews. Genetics·B T LahnK Jegalian
May 12, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mengneng XiongLan Ye
Nov 18, 2000·Journal of Endocrinological Investigation·W M BaarendsJ A Grootegoed
Oct 29, 2003·Journal of Andrology·Alan L Y PangWai-Yee Chan
Nov 1, 2016·Epigenetics & Chromatin·Charlotte MorettiJulie Cocquet
Jul 1, 1997·Mechanisms of Development·W W BranfordS S Potter
Apr 12, 2003·Developmental Cell·Oscar Fernandez-CapetilloAndré Nussenzweig
Apr 22, 2006·Genomics·Violetta KarwackiStephanie Schubert
Mar 21, 2008·Trends in Biochemical Sciences·Marcus GroettrupKay Hofmann

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