Transcriptional analysis of the groES-groEL1, groEL2, and dnaK genes in Corynebacterium glutamicum: characterization of heat shock-induced promoters

Journal of Bacteriology
Carlos BarreiroJuan-Francisco Martín

Abstract

The appropriate conditions to switch on the heat shock promoters in Corynebacterium glutamicum were defined by Northern blot analysis. Transcriptional patterns were characterized for the groEL2 gene and the groES-groEL1 and dnaK operons. Transcriptional start points of these genes were determined by primer extension analysis, allowing the identification of CIRCE and HAIR boxes close to the -10 and -35 regions of the promoters. The presence of both CIRCE and HAIR sequences within a single promoter (P-groEL2) in bacteria is described for the first time. In addition, the dnaK promoter showed -10 and -35 sequences similar to those recognized by SigH of Mycobacterium and SigR of Streptomyces close to a second transcription start region with -10 and -35 boxes typical of promoters for housekeeping genes.

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Citations

Apr 28, 2006·Applied Microbiology and Biotechnology·Juri M SeletzkyJochen Büchs
Nov 19, 2010·Journal of Industrial Microbiology & Biotechnology·Jan Nešvera
Jun 9, 2005·FEMS Microbiology Reviews·Adrianne KlijnFabrizio Arigoni
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Dec 18, 2008·BMC Genomics·Yoon Jung KimYong Keun Chang
Dec 19, 2008·BMC Microbiology·Mónica Barriuso-IglesiasJuan F Martín
Oct 27, 2015·Applied Microbiology and Biotechnology·Koichi Toyoda, Masayuki Inui
Feb 1, 2011·Journal of Biotechnology·Miroslav Pátek, Jan Nešvera
Jan 18, 2007·Journal of Biotechnology·Karina BrinkrolfAndreas Tauch
Jan 12, 2013·Microbial Biotechnology·Miroslav PátekJan Nešvera
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Aug 23, 2005·Biochimica Et Biophysica Acta·Peter HüntenRoland Benz
May 7, 2009·FEMS Microbiology Reviews·Peter A Lund
Apr 27, 2018·Frontiers in Molecular Biosciences·Mohammed Y Ansari, Shekhar C Mande

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