Transcriptional and biochemical analysis of starch metabolism in the hyperthermophilic archaeon Pyrococcus furiosus

Journal of Bacteriology
Han-Seung LeeMichael W W Adams

Abstract

Pyrococcus furiosus utilizes starch and its degradation products, such as maltose, as primary carbon sources, but the pathways by which these alpha-glucans are processed have yet to be defined. For example, its genome contains genes proposed to encode five amylolytic enzymes (including a cyclodextrin glucanotransferase [CGTase] and amylopullulanase), as well as two transporters for maltose and maltodextrins (Mal-I and Mal-II), and a range of intracellular enzymes have been purified that reportedly metabolize maltodextrins and maltose. However, precisely which of these enzymes are involved in starch processing is not clear. In this study, starch metabolism in P. furiosus was examined by biochemical analyses in conjunction with global transcriptional response data for cells grown on a variety of glucans. In addition, DNA sequencing led to the correction of two key errors in the genome sequence, and these change the predicted properties of amylopullulanase (now designated PF1935*) and CGTase (PF0478*). Based on all of these data, a pathway is proposed that is specific for starch utilization that involves one transporter (Mal-II [PF1933 to PF1939]) and only three enzymes, amylopullulanase (PF1935*), 4-alpha-glucanotransferase (PF02...Continue Reading

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Citations

Aug 28, 2013·Extremophiles : Life Under Extreme Conditions·G SchmidM Thomm
Dec 14, 2011·The Journal of Biological Chemistry·Sanjeev K ChandrayanMichael W W Adams
May 29, 2012·Journal of Bacteriology·Stephanie L BridgerMichael W W Adams
Jan 17, 2008·BMC Evolutionary Biology·Kenneth M NollDhaval M Nanavati
Jul 26, 2013·Applied Microbiology and Biotechnology·Jong-Hyun JungCheon-Seok Park
Feb 26, 2011·Archaea : an International Microbiological Journal·Jasper WaltherJohn van der Oost
Jul 24, 2008·Metabolic Engineering·Chung-Jung ChouRobert M Kelly
Apr 2, 2008·Annals of the New York Academy of Sciences·Amy L VanfossenRobert M Kelly
Mar 3, 2011·Biotechnology Progress·Monica C Santa-MariaBryon Sosinski
Jun 6, 2008·Current Opinion in Biotechnology·Sara E Blumer-SchuetteRobert M Kelly
Dec 5, 2014·Extremophiles : Life Under Extreme Conditions·Derrick L LewisRobert M Kelly
Jun 25, 2015·Extremophiles : Life Under Extreme Conditions·Kyoung-Hwa Choi, Jaeho Cha
Jun 23, 2006·FEMS Microbiology Letters·Harmen J G van de WerkenJohn van der Oost
Feb 22, 2017·Biotechnology and Bioengineering·Narendran SekarRamaraja P Ramasamy
May 16, 2019·FEMS Microbiology Letters·Ulrike JohnsenPeter Schönheit
Nov 16, 2018·Extremophiles : Life Under Extreme Conditions·Ana M EstevesHelena Santos

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